「PCR primers cp rbcL」の版間の差分
提供: 広島大学デジタル博物館
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1行目: | 1行目: | ||
− | <span style="font-size:10">[ | + | <span style="font-size:10">[https://www.hiroshima-u.ac.jp/index-j.html 広島大学] > [https://www.digital-museum.hiroshima-u.ac.jp/~museum/ デジタル自然史博物館] > [https://www.digital-museum.hiroshima-u.ac.jp/~museum/plant/ 植物] > [[コケ|コケ植物(蘚苔類)]] > [[コケ/情報|情報]]</span> |
= [[PCR_primers|PCR primers]] = | = [[PCR_primers|PCR primers]] = | ||
== chloroplast == | == chloroplast == | ||
− | === rbcL (atpB-rbcL-trnR-zfpA) === | + | === [[rbcL|''rbcL'' (''atpB''-''rbcL''-''trnR''-''zfpA'')]] === |
{| class="wikitable" style="width:90%; margin:0 2em 0 2em; background-color:#d1d1a5; text-align:left" | {| class="wikitable" style="width:90%; margin:0 2em 0 2em; background-color:#d1d1a5; text-align:left" | ||
− | |+ style="text-align:left" | Table. Sequences (5’–3’) of synthetic primers used for PCR amplification and sequencing of the rbcL gene. The primer numbers from 5’ end correspond to rough positions in the sequence of ''Marchantia polymorpha'' (Ohyama et al. 1986). | + | |+ style="text-align:left" | Table. Sequences (5’–3’) of synthetic primers used for PCR amplification and sequencing of the ''rbcL'' gene. The primer numbers from 5’ end correspond to rough positions in the sequence of ''Marchantia polymorpha'' ([http://www.nature.com/nature/journal/v322/n6079/abs/322572a0.html Ohyama et al. 1986]). |
|- | |- | ||
| Analyzed region | | Analyzed region | ||
14行目: | 14行目: | ||
| For | | For | ||
|- | |- | ||
− | | rbcL | + | | ''atpB''-''rbcL'' |
| Forward | | Forward | ||
| atpB1011 | | atpB1011 | ||
21行目: | 21行目: | ||
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''atpB''-''rbcL'' |
| Forward | | Forward | ||
| atpB370 | | atpB370 | ||
28行目: | 28行目: | ||
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''atpB''-''rbcL'' |
| Forward | | Forward | ||
| atbB175R | | atbB175R | ||
| TGTTGAACTT CACAAGTAAC A | | TGTTGAACTT CACAAGTAAC A | ||
− | | Manhart (1994) | + | | [[Manhart_1994|Manhart (1994)]] |
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
| rbcL−130 | | rbcL−130 | ||
| ACAATGATAC TGTTTGTTAT AG | | ACAATGATAC TGTTTGTTAT AG | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
− | | Bryopytes | + | | Bryopytes, Vascular plants (''Sisymbrium'' etc.) |
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
| rbcL−73 | | rbcL−73 | ||
49行目: | 49行目: | ||
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
− | | rbcL-26Fmas | + | | rbcL−53h |
+ | | TCGAGTAGAC CTTATCCTTG C | ||
+ | | [[Inoue_Tsubota_2014|Inoue & Tsubota (2014)]] | ||
+ | | Bryopytes | ||
+ | |- | ||
+ | | ''rbcL'' | ||
+ | | Forward | ||
+ | | rbcL-26Fmas<ref name="chu1">The PCR primer "rbcL-26Fmas" of Table 1 in [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010a)]] was published with the sequences based on mistranscription of the sequences data, which should be rectified as "5'-TATTAATTGA TTTGTAGGGA GG-3'". In avoiding future confusion we gave a new name to the sequences (rbcL-26Fh: 5'-TATTAATTGA TTTGTAGGGA GG-3').</ref> | ||
| AAGTCCCTCC CTACGAATCAA | | AAGTCCCTCC CTACGAATCAA | ||
| [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | | [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | ||
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
− | | rbcL-26Fh | + | | rbcL-26Fh<ref name="chu1" /> |
− | | TATTAATTGA TTTGTAGGGA GG | + | | TATTAATTGA TTTGTAGGGA GG |
| [[Masuzaki_et_al_2011|Masuzaki et al. (2011)]] | | [[Masuzaki_et_al_2011|Masuzaki et al. (2011)]] | ||
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
| HrL1 | | HrL1 | ||
| ATGTCACCAC AAACGGAGAC TAAAGCAGG | | ATGTCACCAC AAACGGAGAC TAAAGCAGG | ||
| [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | | [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | ||
− | | Bryopytes | + | | Bryopytes, Vascular plants (''Sisymbrium'' etc.) |
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
| rbcL7 | | rbcL7 | ||
| TGGATTTAAA GCTGGTGTTA AAG | | TGGATTTAAA GCTGGTGTTA AAG | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
− | | Bryopytes | + | | Bryopytes, Vascular plants (''Sisymbrium'' etc.) |
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
| rbcL39Fmas | | rbcL39Fmas | ||
84行目: | 91行目: | ||
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
| rbcL111Fmas | | rbcL111Fmas | ||
91行目: | 98行目: | ||
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
| rbcL152 | | rbcL152 | ||
98行目: | 105行目: | ||
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
| rbcL218h | | rbcL218h | ||
| GACGATGCTA TGATCTCGAA GC | | GACGATGCTA TGATCTCGAA GC | ||
− | | Tsubota et al. (2000) | + | | [[Tsubota_Nakao_Yamaguchi_Seki_Deguchi_2000|Tsubota et al. (2000)]] |
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
| rbcL256 | | rbcL256 | ||
| GCTATGATCT TGAAGCAGTT CCTGGAGAAG | | GCTATGATCT TGAAGCAGTT CCTGGAGAAG | ||
− | | Tsubota et al. (2000) | + | | [[Tsubota_Nakao_Yamaguchi_Seki_Deguchi_2000|Tsubota et al. (2000)]] |
+ | | Bryopytes | ||
+ | |- | ||
+ | | ''rbcL'' | ||
+ | | Forward | ||
+ | | rbcL333mas | ||
+ | | AATCAATATA TTGCTTATGT | ||
+ | | [[Masuzaki_et_al_2011|Masuzaki et al. (2011)]] | ||
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
| rbcL265 | | rbcL265 | ||
119行目: | 133行目: | ||
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
| rbcL557 | | rbcL557 | ||
| GGTAGAGCTG TATATGAATG TCTTCGTGGT GG | | GGTAGAGCTG TATATGAATG TCTTCGTGGT GG | ||
− | | Tsubota et al. (2000) | + | | [[Tsubota_Nakao_Yamaguchi_Seki_Deguchi_2000|Tsubota et al. (2000)]] |
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
| rbcL549 | | rbcL549 | ||
| TGTCTTCGTG GTGGAC | | TGTCTTCGTG GTGGAC | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
− | | Bryopytes | + | | Bryopytes, Vascular plants (''Sisymbrium'' etc.) |
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
| rbcL634Fmas | | rbcL634Fmas | ||
140行目: | 154行目: | ||
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
| rbcL862 | | rbcL862 | ||
| CAATGCATGC AGTTATTGAC | | CAATGCATGC AGTTATTGAC | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
− | | Bryopytes | + | | Bryopytes, Vascular plants (''Sisymbrium'' etc.) |
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
| rbcL919G | | rbcL919G | ||
| CATGGTATGC ATTTCCGTGT A | | CATGGTATGC ATTTCCGTGT A | ||
− | | Tsubota et al. (2001) | + | | [[Tsubota_Akiyama_Yamaguchi_Deguchi_2001|Tsubota et al. (2001)]] |
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
| rbcL920Fmas | | rbcL920Fmas | ||
161行目: | 175行目: | ||
| Bryopytes (Liverworts) | | Bryopytes (Liverworts) | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
| rbcL921G | | rbcL921G | ||
| GGTATGCATT TCCGTGTATT AGC | | GGTATGCATT TCCGTGTATT AGC | ||
− | | Tsubota et al. (2001) | + | | [[Tsubota_Akiyama_Yamaguchi_Deguchi_2001|Tsubota et al. (2001)]] |
− | | Bryopytes | + | | Bryopytes, Vascular plants (''Sisymbrium'' etc.) |
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
| rbcL968G | | rbcL968G | ||
| GAGATCATAT TCACGCTGGT AC | | GAGATCATAT TCACGCTGGT AC | ||
− | | Tsubota et al. (2001) | + | | [[Tsubota_Akiyama_Yamaguchi_Deguchi_2001|Tsubota et al. (2001)]] |
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
| rbcL1117 | | rbcL1117 | ||
| GGTATTCATG TTTGGC | | GGTATTCATG TTTGGC | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
− | | Bryopytes | + | | Bryopytes, Vascular plants (''Sisymbrium'' etc.) |
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
| rbcL1220 | | rbcL1220 | ||
189行目: | 203行目: | ||
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Forward | | Forward | ||
| rbcL1276 | | rbcL1276 | ||
203行目: | 217行目: | ||
| | | | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL''-''trnR''-''zfpA'' |
| Reverse | | Reverse | ||
| zfpA546R | | zfpA546R | ||
| ATGATATCCA CATTCTTCAC A | | ATGATATCCA CATTCTTCAC A | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
− | | Bryopytes | + | | Bryopytes, Vascular plants (''Sisymbrium'' etc.) |
|- | |- | ||
− | | rbcL | + | | ''rbcL''-''trnR'' |
| Reverse | | Reverse | ||
| trnRn | | trnRn | ||
| GGGTTAGAAG GGATTCGAAC CCTTGAC | | GGGTTAGAAG GGATTCGAAC CCTTGAC | ||
− | | Manhart (1994); [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | + | | [[Manhart_1994|Manhart (1994)]]; [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] |
− | | Bryopytes | + | | Bryopytes, Vascular plants (''Sisymbrium'' etc.) |
|- | |- | ||
− | | rbcL | + | | ''rbcL''-''trnR'' |
| Reverse | | Reverse | ||
| trnR66R | | trnR66R | ||
224行目: | 238行目: | ||
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL''-''trnR'' |
| Reverse | | Reverse | ||
| trnR24R | | trnR24R | ||
231行目: | 245行目: | ||
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Reverse | | Reverse | ||
| rbcL1346hR | | rbcL1346hR | ||
238行目: | 252行目: | ||
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Reverse | | Reverse | ||
| rbcL1301RL | | rbcL1301RL | ||
| CTTCATTACG TGCTTGTACA CAAGCTTCTA | | CTTCATTACG TGCTTGTACA CAAGCTTCTA | ||
| [[Inoue_et_al_2011|Inoue et al. (2011)]] | | [[Inoue_et_al_2011|Inoue et al. (2011)]] | ||
− | | Bryopytes | + | | Bryopytes, Vascular plants (''Sisymbrium'' etc.) |
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Reverse | | Reverse | ||
| rbcL1145R | | rbcL1145R | ||
| TTAATGCTGG CATATGCCAA AC | | TTAATGCTGG CATATGCCAA AC | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
− | | Bryopytes | + | | Bryopytes, Vascular plants (''Sisymbrium'' etc.) |
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Reverse | | Reverse | ||
| rbcL1098R | | rbcL1098R | ||
259行目: | 273行目: | ||
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Reverse | | Reverse | ||
| ArbcL-MR | | ArbcL-MR | ||
266行目: | 280行目: | ||
| Ferns, Bryopytes | | Ferns, Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Reverse | | Reverse | ||
| rbcL989R | | rbcL989R | ||
| CTACGGTACC AGCGTGAATA TGGTCTCCAC CGG | | CTACGGTACC AGCGTGAATA TGGTCTCCAC CGG | ||
− | | Tsubota et al. (2000) | + | | [[Tsubota_Nakao_Yamaguchi_Seki_Deguchi_2000|Tsubota et al. (2000)]] |
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Reverse | | Reverse | ||
| rbcL957R | | rbcL957R | ||
280行目: | 294行目: | ||
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
+ | | Reverse | ||
+ | | HrL911R<ref name="chu4">Masuzaki et al.(2011)のHrL911Rの配列は5'-CGTGCAATGC ATGCAGTTAT TGATAGACA-3'となっているが,実際に用いたものはHrL911Rtmの配列.</ref> | ||
+ | | CGTGCAATGC ATGCAGTTAT TGATAGACA | ||
+ | | [[Masuzaki_et_al_2011|Masuzaki et al. (2011)]] | ||
+ | | Bryopytes | ||
+ | |- | ||
+ | | ''rbcL'' | ||
+ | | Reverse | ||
+ | | HrL911Rtm<ref name="chu6">Masuzaki et al.(2011)の配列を訂正</ref> | ||
+ | | TGTCTATCAA TAACTGCATG CATTGCACG | ||
+ | | [[Phan_Nakahara-Tsubota_Inoue_Tsubota_2021|Phan et al. (2021)]] | ||
+ | | Bryopytes, Vascular plants (''Dysphania'' etc.) | ||
+ | |- | ||
+ | | ''rbcL'' | ||
| Reverse | | Reverse | ||
| rbcL804hR | | rbcL804hR | ||
| TGCAGTAAAA CCACCTG | | TGCAGTAAAA CCACCTG | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
− | | Bryopytes | + | | Bryopytes, Vascular plants (''Sisymbrium'' etc.) |
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
− | | | + | | Reverse |
| rbcL650Rmas | | rbcL650Rmas | ||
| CGATCTCTCC AACGCA | | CGATCTCTCC AACGCA | ||
| [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | | [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | ||
− | | Bryopytes | + | | Bryopytes, Vascular plants (''Sisymbrium'' etc.) |
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Reverse | | Reverse | ||
− | | rbcL600R | + | | rbcL600R<ref name="chu2">Masuzaki et al. (2011)のrbcL600Rの配列は5'-CGTGGTGGAC TTGATTTCAC-3'となっているが,誤り.</ref> |
| GTGAAATCAA GTCCACCACG | | GTGAAATCAA GTCCACCACG | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
− | | Bryopytes | + | | Bryopytes, Vascular plants (''Sisymbrium'' etc.) |
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
− | | | + | | Reverse |
| rbcL302Rmas | | rbcL302Rmas | ||
| ACATAAGCAA TATATTGATT | | ACATAAGCAA TATATTGATT | ||
308行目: | 336行目: | ||
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
+ | | Reverse | ||
+ | | HrL260R<ref name="chu3">Inoue et al. (2011)では,Masuzaki et al. (2010)が初出となっているが,実際にはInoue et al. (2011)が初出.</ref><ref name="chu5">The first appearance of this primer was in Table 1 of Inoue et al. (2011), and the footnote in Inoue et al. (2011) should be requested to make the corrections as the following: correct: “(published here)” and incorrect: “published by Masuzaki et al. (2010)”.</ref> | ||
+ | | ATATCATAGC ATCGACCTTT GTAACGATC | ||
+ | | [[Inoue_et_al_2011|Inoue et al. (2011)]] | ||
+ | | Bryopytes, Vascular plants (''Sisymbrium'' etc.) | ||
+ | |- | ||
+ | | ''rbcL'' | ||
| Reverse | | Reverse | ||
| rbcL270R | | rbcL270R | ||
315行目: | 350行目: | ||
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
| Reverse | | Reverse | ||
| rbcL199R | | rbcL199R | ||
322行目: | 357行目: | ||
| Bryopytes | | Bryopytes | ||
|- | |- | ||
− | | rbcL | + | | ''rbcL'' |
− | | | + | | Reverse |
| rbcL128Rmas | | rbcL128Rmas | ||
| GTCATACGAA ATGCTGC | | GTCATACGAA ATGCTGC | ||
330行目: | 365行目: | ||
|} | |} | ||
− | + | == 脚注 == | |
− | + | {{Reflist|2}} | |
− | = | + | =[[テンプレート:PCR_primers_References|References]]= |
− | + | {{PCR_primers_References}} | |
− | |||
− | |||
− | |||
+ | ==[[テンプレート:primers|関連ページ]]== | ||
+ | {{primers}} | ||
---- | ---- | ||
− | [ | + | [https://www.digital-museum.hiroshima-u.ac.jp/~museum/ デジタル自然史博物館] / [https://www.hiroshima-u.ac.jp 広島大学] / [https://www.digital-museum.hiroshima-u.ac.jp/~museum/plant/ 植物] / [[コケ|コケ植物(蘚苔類)]] / [[コケ/情報|情報]] にもどる |
[[Category:広島大学デジタル自然史博物館]] | [[Category:広島大学デジタル自然史博物館]] | ||
349行目: | 383行目: | ||
[[Category:研究]] | [[Category:研究]] | ||
[[Category:PCR]] | [[Category:PCR]] | ||
+ | [[Category:primer]] | ||
[[Category:系統]] | [[Category:系統]] | ||
[[Category:生物]] | [[Category:生物]] | ||
+ | [[Category:rbcL]] | ||
+ | [[Category:葉緑体]] |
2022年5月3日 (火) 15:37時点における版
広島大学 > デジタル自然史博物館 > 植物 > コケ植物(蘚苔類) > 情報
PCR primers
chloroplast
rbcL (atpB-rbcL-trnR-zfpA)
Analyzed region | Forward / Reverse | Primer name | Sequence (5'–3') | References | For |
atpB-rbcL | Forward | atpB1011 | GATCTACTAC TTTAATTCCT GT | Tsubota et al. (1999) | Bryopytes |
atpB-rbcL | Forward | atpB370 | AGTTGAATCT AAAGGATCTA C | Tsubota et al. (1999) | Bryopytes |
atpB-rbcL | Forward | atbB175R | TGTTGAACTT CACAAGTAAC A | Manhart (1994) | Bryopytes |
rbcL | Forward | rbcL−130 | ACAATGATAC TGTTTGTTAT AG | Tsubota et al. (1999) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Forward | rbcL−73 | ATACCAAAGA TGTTTTTTTA TAAG | Tsubota et al. (1999) | Bryopytes |
rbcL | Forward | rbcL−53h | TCGAGTAGAC CTTATCCTTG C | Inoue & Tsubota (2014) | Bryopytes |
rbcL | Forward | rbcL-26Fmas[1] | AAGTCCCTCC CTACGAATCAA | Masuzaki et al. (2010) | Bryopytes |
rbcL | Forward | rbcL-26Fh[1] | TATTAATTGA TTTGTAGGGA GG | Masuzaki et al. (2011) | Bryopytes |
rbcL | Forward | HrL1 | ATGTCACCAC AAACGGAGAC TAAAGCAGG | Masuzaki et al. (2010) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Forward | rbcL7 | TGGATTTAAA GCTGGTGTTA AAG | Tsubota et al. (1999) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Forward | rbcL39Fmas | AAAGCTGGTG TTAAAGATTA | Masuzaki et al. (2010) | Bryopytes |
rbcL | Forward | rbcL111Fmas | GCAGCATTTC GTATGAC | Masuzaki et al. (2010) | Bryopytes |
rbcL | Forward | rbcL152 | GAATCCTCCA CTGGTACATG | Tsubota et al. (1999) | Bryopytes |
rbcL | Forward | rbcL218h | GACGATGCTA TGATCTCGAA GC | Tsubota et al. (2000) | Bryopytes |
rbcL | Forward | rbcL256 | GCTATGATCT TGAAGCAGTT CCTGGAGAAG | Tsubota et al. (2000) | Bryopytes |
rbcL | Forward | rbcL333mas | AATCAATATA TTGCTTATGT | Masuzaki et al. (2011) | Bryopytes |
rbcL | Forward | rbcL265 | ATTGCTTATG TTGCTTATCC | Tsubota et al. (1999) | Bryopytes |
rbcL | Forward | rbcL557 | GGTAGAGCTG TATATGAATG TCTTCGTGGT GG | Tsubota et al. (2000) | Bryopytes |
rbcL | Forward | rbcL549 | TGTCTTCGTG GTGGAC | Tsubota et al. (1999) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Forward | rbcL634Fmas | ATGCGTTGGA GAGA | Masuzaki et al. (2010) | Bryopytes |
rbcL | Forward | rbcL862 | CAATGCATGC AGTTATTGAC | Tsubota et al. (1999) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Forward | rbcL919G | CATGGTATGC ATTTCCGTGT A | Tsubota et al. (2001) | Bryopytes |
rbcL | Forward | rbcL920Fmas | CATGGTATGC ATTTCCGTGT | Masuzaki et al. (2010) | Bryopytes (Liverworts) |
rbcL | Forward | rbcL921G | GGTATGCATT TCCGTGTATT AGC | Tsubota et al. (2001) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Forward | rbcL968G | GAGATCATAT TCACGCTGGT AC | Tsubota et al. (2001) | Bryopytes |
rbcL | Forward | rbcL1117 | GGTATTCATG TTTGGC | Tsubota et al. (1999) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Forward | rbcL1220 | GGAGCAGTTG CTAATAGAG | Tsubota et al. (1999) | Bryopytes |
rbcL | Forward | rbcL1276 | GGACGTGATC TTGCTCG | Tsubota et al. (1999) | Bryopytes |
rbcL-trnR-zfpA | Reverse | zfpA546R | ATGATATCCA CATTCTTCAC A | Tsubota et al. (1999) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL-trnR | Reverse | trnRn | GGGTTAGAAG GGATTCGAAC CCTTGAC | Manhart (1994); Tsubota et al. (1999) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL-trnR | Reverse | trnR66R | GAAGGGATTC GAACCCTTG | Tsubota et al. (1999) | Bryopytes |
rbcL-trnR | Reverse | trnR24R | CTCTAATCCA CTGAGCTACA | Tsubota et al. (1999) | Bryopytes |
rbcL | Reverse | rbcL1346hR | GCAGCTAATT CAGGACTCC | Tsubota et al. (1999) | Bryopytes |
rbcL | Reverse | rbcL1301RL | CTTCATTACG TGCTTGTACA CAAGCTTCTA | Inoue et al. (2011) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Reverse | rbcL1145R | TTAATGCTGG CATATGCCAA AC | Tsubota et al. (1999) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Reverse | rbcL1098R | AACACCTGGT AAAGAAACC | Tsubota et al. (1999) | Bryopytes |
rbcL | Reverse | ArbcL-MR | TGACGTTCTC CTTCAAGTTT ACCTACTAC | Murakami et al. (1999) | Ferns, Bryopytes |
rbcL | Reverse | rbcL989R | CTACGGTACC AGCGTGAATA TGGTCTCCAC CGG | Tsubota et al. (2000) | Bryopytes |
rbcL | Reverse | rbcL957R | GCGTGAATAT GGTCTCCACC | Tsubota et al. (1999) | Bryopytes |
rbcL | Reverse | HrL911R[2] | CGTGCAATGC ATGCAGTTAT TGATAGACA | Masuzaki et al. (2011) | Bryopytes |
rbcL | Reverse | HrL911Rtm[3] | TGTCTATCAA TAACTGCATG CATTGCACG | Phan et al. (2021) | Bryopytes, Vascular plants (Dysphania etc.) |
rbcL | Reverse | rbcL804hR | TGCAGTAAAA CCACCTG | Tsubota et al. (1999) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Reverse | rbcL650Rmas | CGATCTCTCC AACGCA | Masuzaki et al. (2010) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Reverse | rbcL600R[4] | GTGAAATCAA GTCCACCACG | Tsubota et al. (1999) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Reverse | rbcL302Rmas | ACATAAGCAA TATATTGATT | Masuzaki et al. (2010) | Bryopytes |
rbcL | Reverse | HrL260R[5][6] | ATATCATAGC ATCGACCTTT GTAACGATC | Inoue et al. (2011) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Reverse | rbcL270R | GCAATATATT GATTTTCTTC TCCAG | Tsubota et al. (1999) | Bryopytes |
rbcL | Reverse | rbcL199R | GGTAAGTCCA TCAGTCCAAA C | Tsubota et al. (1999) | Bryopytes |
rbcL | Reverse | rbcL128Rmas | GTCATACGAA ATGCTGC | Masuzaki et al. (2010) | Bryopytes |
脚注
- ↑ 1.0 1.1 The PCR primer "rbcL-26Fmas" of Table 1 in Masuzaki et al. (2010a) was published with the sequences based on mistranscription of the sequences data, which should be rectified as "5'-TATTAATTGA TTTGTAGGGA GG-3'". In avoiding future confusion we gave a new name to the sequences (rbcL-26Fh: 5'-TATTAATTGA TTTGTAGGGA GG-3').
- ↑ Masuzaki et al.(2011)のHrL911Rの配列は5'-CGTGCAATGC ATGCAGTTAT TGATAGACA-3'となっているが,実際に用いたものはHrL911Rtmの配列.
- ↑ Masuzaki et al.(2011)の配列を訂正
- ↑ Masuzaki et al. (2011)のrbcL600Rの配列は5'-CGTGGTGGAC TTGATTTCAC-3'となっているが,誤り.
- ↑ Inoue et al. (2011)では,Masuzaki et al. (2010)が初出となっているが,実際にはInoue et al. (2011)が初出.
- ↑ The first appearance of this primer was in Table 1 of Inoue et al. (2011), and the footnote in Inoue et al. (2011) should be requested to make the corrections as the following: correct: “(published here)” and incorrect: “published by Masuzaki et al. (2010)”.
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関連ページ
- UBC primers
- IUPAC/UBMB codes for nucleic acid bases [Nucleotide base codes (IUPAC)]
- Nucleotide base codes
- pyrimidine
- purine
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