「PCR primers cp rbcL」の版間の差分

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25行目: 25行目:
 
| atpB370
 
| atpB370
 
| AGTTGAATCT AAAGGATCTA C
 
| AGTTGAATCT AAAGGATCTA C
−
| Tsubota et al. (1999)
+
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|-
 
|-
39行目: 39行目:
 
| rbcL−130
 
| rbcL−130
 
| ACAATGATAC TGTTTGTTAT AG
 
| ACAATGATAC TGTTTGTTAT AG
−
| Tsubota et al. (1999)
+
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|-
 
|-
46行目: 46行目:
 
| rbcL−73
 
| rbcL−73
 
| ATACCAAAGA TGTTTTTTTA TAAG
 
| ATACCAAAGA TGTTTTTTTA TAAG
−
| Tsubota et al. (1999)
+
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|-
 
|-
53行目: 53行目:
 
| rbcL7
 
| rbcL7
 
| TGGATTTAAA GCTGGTGTTA AAG
 
| TGGATTTAAA GCTGGTGTTA AAG
−
| Tsubota et al. (1999)
+
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|-
 
|-
67行目: 67行目:
 
| rbcL152
 
| rbcL152
 
| GAATCCTCCA CTGGTACATG
 
| GAATCCTCCA CTGGTACATG
−
| Tsubota et al. (1999)
+
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|-
 
|-
88行目: 88行目:
 
| rbcL265
 
| rbcL265
 
| ATTGCTTATG TTGCTTATCC
 
| ATTGCTTATG TTGCTTATCC
−
| Tsubota et al. (1999)
+
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|-
 
|-
102行目: 102行目:
 
| rbcL549
 
| rbcL549
 
| TGTCTTCGTG GTGGAC
 
| TGTCTTCGTG GTGGAC
−
| Tsubota et al. (1999)
+
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|-
 
|-
109行目: 109行目:
 
| rbcL862
 
| rbcL862
 
| CAATGCATGC AGTTATTGAC
 
| CAATGCATGC AGTTATTGAC
−
| Tsubota et al. (1999)
+
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|-
 
|-
137行目: 137行目:
 
| rbcL1117
 
| rbcL1117
 
| GGTATTCATG TTTGGC
 
| GGTATTCATG TTTGGC
−
| Tsubota et al. (1999)
+
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|-
 
|-
144行目: 144行目:
 
| rbcL1220
 
| rbcL1220
 
| GGAGCAGTTG CTAATAGAG
 
| GGAGCAGTTG CTAATAGAG
−
| Tsubota et al. (1999)
+
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|-
 
|-
151行目: 151行目:
 
| rbcL1276
 
| rbcL1276
 
| GGACGTGATC TTGCTCG
 
| GGACGTGATC TTGCTCG
−
| Tsubota et al. (1999)
+
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|-
 
|-
165行目: 165行目:
 
| zfpA546R
 
| zfpA546R
 
| ATGATATCCA CATTCTTCAC A
 
| ATGATATCCA CATTCTTCAC A
−
| Tsubota et al. (1999)
+
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|-
 
|-
172行目: 172行目:
 
| trnRn
 
| trnRn
 
| GGGTTAGAAG GGATTCGAAC CCTTGAC
 
| GGGTTAGAAG GGATTCGAAC CCTTGAC
−
| Manhart (1994); Tsubota et al. (1999)
+
| Manhart (1994); [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|-
 
|-
179行目: 179行目:
 
| trnR66R
 
| trnR66R
 
| GAAGGGATTC GAACCCTTG
 
| GAAGGGATTC GAACCCTTG
−
| Tsubota et al. (1999)
+
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|-
 
|-
186行目: 186行目:
 
| trnR24R
 
| trnR24R
 
| CTCTAATCCA CTGAGCTACA
 
| CTCTAATCCA CTGAGCTACA
−
| Tsubota et al. (1999)
+
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|-
 
|-
193行目: 193行目:
 
| rbcL1346hR
 
| rbcL1346hR
 
| GCAGCTAATT CAGGACTCC
 
| GCAGCTAATT CAGGACTCC
−
| Tsubota et al. (1999)
+
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|-
 
|-
207行目: 207行目:
 
| rbcL1145R
 
| rbcL1145R
 
| TTAATGCTGG CATATGCCAA AC
 
| TTAATGCTGG CATATGCCAA AC
−
| Tsubota et al. (1999)
+
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|-
 
|-
214行目: 214行目:
 
| rbcL1098R
 
| rbcL1098R
 
| AACACCTGGT AAAGAAACC
 
| AACACCTGGT AAAGAAACC
−
| Tsubota et al. (1999)
+
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|-
 
|-
235行目: 235行目:
 
| rbcL957R
 
| rbcL957R
 
| GCGTGAATAT GGTCTCCACC
 
| GCGTGAATAT GGTCTCCACC
−
| Tsubota et al. (1999)
+
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|-
 
|-
242行目: 242行目:
 
| rbcL804hR
 
| rbcL804hR
 
| TGCAGTAAAA CCACCTG
 
| TGCAGTAAAA CCACCTG
−
| Tsubota et al. (1999)
+
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|-
 
|-
249行目: 249行目:
 
| rbcL600R
 
| rbcL600R
 
| GTGAAATCAA GTCCACCACG
 
| GTGAAATCAA GTCCACCACG
−
| Tsubota et al. (1999)
+
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|-
 
|-
256行目: 256行目:
 
| rbcL270R
 
| rbcL270R
 
| GCAATATATT GATTTTCTTC TCCAG
 
| GCAATATATT GATTTTCTTC TCCAG
−
| Tsubota et al. (1999)
+
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|-
 
|-
263行目: 263行目:
 
| rbcL199R
 
| rbcL199R
 
| GGTAAGTCCA TCAGTCCAAA C
 
| GGTAAGTCCA TCAGTCCAAA C
−
| Tsubota et al. (1999)
+
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Bryopytes
 
| Bryopytes
 
|}
 
|}

2013年8月3日 (土) 13:06時点における版

広島大学 > デジタル自然史博物館 > 植物 > コケ植物（蘚苔類） > 情報

PCR primers

chloroplast

rbcL

Table. Sequences (5’–3’) of synthetic primers used for PCR amplification and sequencing of the rbcL gene. The primer numbers from 5’ end correspond to rough positions in the sequence of Marchantia polymorpha (Ohyama et al. 1986).
Analyzed region Forward / Reverse Primer name Sequence (5'–3') References For
rbcL Forward atpB1011 GATCTACTAC TTTAATTCCT GT Tsubota et al. (1999) Bryopytes
rbcL Forward atpB370 AGTTGAATCT AAAGGATCTA C Tsubota et al. (1999) Bryopytes
rbcL Forward atbB175R TGTTGAACTT CACAAGTAAC A Manhart (1994) Bryopytes
rbcL Forward rbcL−130 ACAATGATAC TGTTTGTTAT AG Tsubota et al. (1999) Bryopytes
rbcL Forward rbcL−73 ATACCAAAGA TGTTTTTTTA TAAG Tsubota et al. (1999) Bryopytes
rbcL Forward rbcL7 TGGATTTAAA GCTGGTGTTA AAG Tsubota et al. (1999) Bryopytes
rbcL Forward HrL1 ATGTCACCAC AAACGGAGAC TAAAGCAGG Masuzaki et al. (2010) Bryopytes
rbcL Forward rbcL152 GAATCCTCCA CTGGTACATG Tsubota et al. (1999) Bryopytes
rbcL Forward rbcL218h GACGATGCTA TGATCTCGAA GC Tsubota et al. (2000) Bryopytes
rbcL Forward rbcL256 GCTATGATCT TGAAGCAGTT CCTGGAGAAG Tsubota et al. (2000) Bryopytes
rbcL Forward rbcL265 ATTGCTTATG TTGCTTATCC Tsubota et al. (1999) Bryopytes
rbcL Forward rbcL557 GGTAGAGCTG TATATGAATG TCTTCGTGGT GG Tsubota et al. (2000) Bryopytes
rbcL Forward rbcL549 TGTCTTCGTG GTGGAC Tsubota et al. (1999) Bryopytes
rbcL Forward rbcL862 CAATGCATGC AGTTATTGAC Tsubota et al. (1999) Bryopytes
rbcL Forward rbcL919G CATGGTATGC ATTTCCGTGT A Tsubota et al. (2001) Bryopytes
rbcL Forward rbcL921G GGTATGCATT TCCGTGTATT AGC Tsubota et al. (2001) Bryopytes
rbcL Forward rbcL968G GAGATCATAT TCACGCTGGT AC Tsubota et al. (2001) Bryopytes
rbcL Forward rbcL1117 GGTATTCATG TTTGGC Tsubota et al. (1999) Bryopytes
rbcL Forward rbcL1220 GGAGCAGTTG CTAATAGAG Tsubota et al. (1999) Bryopytes
rbcL Forward rbcL1276 GGACGTGATC TTGCTCG Tsubota et al. (1999) Bryopytes
rbcL Reverse zfpA546R ATGATATCCA CATTCTTCAC A Tsubota et al. (1999) Bryopytes
rbcL Reverse trnRn GGGTTAGAAG GGATTCGAAC CCTTGAC Manhart (1994); Tsubota et al. (1999) Bryopytes
rbcL Reverse trnR66R GAAGGGATTC GAACCCTTG Tsubota et al. (1999) Bryopytes
rbcL Reverse trnR24R CTCTAATCCA CTGAGCTACA Tsubota et al. (1999) Bryopytes
rbcL Reverse rbcL1346hR GCAGCTAATT CAGGACTCC Tsubota et al. (1999) Bryopytes
rbcL Reverse rbcL1301RL CTTCATTACG TGCTTGTACA CAAGCTTCTA Inoue et al. (2011) Bryopytes
rbcL Reverse rbcL1145R TTAATGCTGG CATATGCCAA AC Tsubota et al. (1999) Bryopytes
rbcL Reverse rbcL1098R AACACCTGGT AAAGAAACC Tsubota et al. (1999) Bryopytes
rbcL Reverse ArbcL-MR TGACGTTCTC CTTCAAGTTT ACCTACTAC Murakami et al. (1999) Ferns, Bryopytes
rbcL Reverse rbcL989R CTACGGTACC AGCGTGAATA TGGTCTCCAC CGG Tsubota et al. (2000) Bryopytes
rbcL Reverse rbcL957R GCGTGAATAT GGTCTCCACC Tsubota et al. (1999) Bryopytes
rbcL Reverse rbcL804hR TGCAGTAAAA CCACCTG Tsubota et al. (1999) Bryopytes
rbcL Reverse rbcL600R GTGAAATCAA GTCCACCACG Tsubota et al. (1999) Bryopytes
rbcL Reverse rbcL270R GCAATATATT GATTTTCTTC TCCAG Tsubota et al. (1999) Bryopytes
rbcL Reverse rbcL199R GGTAAGTCCA TCAGTCCAAA C Tsubota et al. (1999) Bryopytes

References

  • Tsubota, H., N. Nakao, T. Arikawa, T. Yamaguchi, M. Higuchi, H. Deguchi & T. Seki. 1999. A preliminary phylogeny of Hypnales (Musci) as inferred from chloroplast rbcL sequence data. Bryol. Res. 7: 233-248. pdf
  • Tsubota, H., N. Nakao, T. Yamaguchi, T. Seki & H. Deguchi. 2000. Preliminary phylogenetic relationships of the genus Brotherella and its allied genera (Hypnales, Musci) based on chloroplast rbcL sequence data. J. Hattori Bot. Lab. 88: 79-99. [[[Abstract]>http://sciencelinks.jp/j-east/article/200021/000020002100A0795866.php]]
  • Tsubota, H., H. Akiyama, T. Yamaguchi & H. Deguchi. 2001. Molecular phylogeny of the Sematophyllaceae (Hypnales, Musci) based on chloroplast rbcL sequences. J. Hattori Bot. Lab. 90: 221-240.

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