PCR primers cp rbcL

広島大学 > デジタル自然史博物館 > 植物 > コケ植物（蘚苔類） > 情報

PCR primers

chloroplast

rbcL (atpB-rbcL-trnR-zfpA)

Table. Sequences (5’–3’) of synthetic primers used for PCR amplification and sequencing of the rbcL gene. The primer numbers from 5’ end correspond to rough positions in the sequence of Marchantia polymorpha (Ohyama et al. 1986).

Analyzed region	Forward / Reverse	Primer name	Sequence (5'–3')	References	For
rbcL	Forward	atpB1011	GATCTACTAC TTTAATTCCT GT	Tsubota et al. (1999)	Bryopytes
rbcL	Forward	atpB370	AGTTGAATCT AAAGGATCTA C	Tsubota et al. (1999)	Bryopytes
rbcL	Forward	atbB175R	TGTTGAACTT CACAAGTAAC A	Manhart (1994)	Bryopytes
rbcL	Forward	rbcL−130	ACAATGATAC TGTTTGTTAT AG	Tsubota et al. (1999)	Bryopytes
rbcL	Forward	rbcL−73	ATACCAAAGA TGTTTTTTTA TAAG	Tsubota et al. (1999)	Bryopytes
rbcL	Forward	rbcL-26Fmas *	AAGTCCCTCC CTACGAATCAA	Masuzaki et al. (2010)	Bryopytes
rbcL	Forward	rbcL-26Fh	TATTAATTGA TTTGTAGGGA GG *	Masuzaki et al. (2011)	Bryopytes
rbcL	Forward	HrL1	ATGTCACCAC AAACGGAGAC TAAAGCAGG	Masuzaki et al. (2010)	Bryopytes
rbcL	Forward	rbcL7	TGGATTTAAA GCTGGTGTTA AAG	Tsubota et al. (1999)	Bryopytes
rbcL	Forward	rbcL39Fmas	AAAGCTGGTG TTAAAGATTA	Masuzaki et al. (2010)	Bryopytes
rbcL	Forward	rbcL111Fmas	GCAGCATTTC GTATGAC	Masuzaki et al. (2010)	Bryopytes
rbcL	Forward	rbcL152	GAATCCTCCA CTGGTACATG	Tsubota et al. (1999)	Bryopytes
rbcL	Forward	rbcL218h	GACGATGCTA TGATCTCGAA GC	Tsubota et al. (2000)	Bryopytes
rbcL	Forward	rbcL256	GCTATGATCT TGAAGCAGTT CCTGGAGAAG	Tsubota et al. (2000)	Bryopytes
rbcL	Forward	rbcL265	ATTGCTTATG TTGCTTATCC	Tsubota et al. (1999)	Bryopytes
rbcL	Forward	rbcL557	GGTAGAGCTG TATATGAATG TCTTCGTGGT GG	Tsubota et al. (2000)	Bryopytes
rbcL	Forward	rbcL549	TGTCTTCGTG GTGGAC	Tsubota et al. (1999)	Bryopytes
rbcL	Forward	rbcL634Fmas	ATGCGTTGGA GAGA	Masuzaki et al. (2010)	Bryopytes
rbcL	Forward	rbcL862	CAATGCATGC AGTTATTGAC	Tsubota et al. (1999)	Bryopytes
rbcL	Forward	rbcL919G	CATGGTATGC ATTTCCGTGT A	Tsubota et al. (2001)	Bryopytes
rbcL	Forward	rbcL920Fmas	CATGGTATGC ATTTCCGTGT	Masuzaki et al. (2010)	Bryopytes (Liverworts)
rbcL	Forward	rbcL921G	GGTATGCATT TCCGTGTATT AGC	Tsubota et al. (2001)	Bryopytes
rbcL	Forward	rbcL968G	GAGATCATAT TCACGCTGGT AC	Tsubota et al. (2001)	Bryopytes
rbcL	Forward	rbcL1117	GGTATTCATG TTTGGC	Tsubota et al. (1999)	Bryopytes
rbcL	Forward	rbcL1220	GGAGCAGTTG CTAATAGAG	Tsubota et al. (1999)	Bryopytes
rbcL	Forward	rbcL1276	GGACGTGATC TTGCTCG	Tsubota et al. (1999)	Bryopytes
rbcL	Reverse	zfpA546R	ATGATATCCA CATTCTTCAC A	Tsubota et al. (1999)	Bryopytes
rbcL	Reverse	trnRn	GGGTTAGAAG GGATTCGAAC CCTTGAC	Manhart (1994); Tsubota et al. (1999)	Bryopytes
rbcL	Reverse	trnR66R	GAAGGGATTC GAACCCTTG	Tsubota et al. (1999)	Bryopytes
rbcL	Reverse	trnR24R	CTCTAATCCA CTGAGCTACA	Tsubota et al. (1999)	Bryopytes
rbcL	Reverse	rbcL1346hR	GCAGCTAATT CAGGACTCC	Tsubota et al. (1999)	Bryopytes
rbcL	Reverse	rbcL1301RL	CTTCATTACG TGCTTGTACA CAAGCTTCTA	Inoue et al. (2011)	Bryopytes
rbcL	Reverse	rbcL1145R	TTAATGCTGG CATATGCCAA AC	Tsubota et al. (1999)	Bryopytes
rbcL	Reverse	rbcL1098R	AACACCTGGT AAAGAAACC	Tsubota et al. (1999)	Bryopytes
rbcL	Reverse	ArbcL-MR	TGACGTTCTC CTTCAAGTTT ACCTACTAC	Murakami et al. (1999)	Ferns, Bryopytes
rbcL	Reverse	rbcL989R	CTACGGTACC AGCGTGAATA TGGTCTCCAC CGG	Tsubota et al. (2000)	Bryopytes
rbcL	Reverse	rbcL957R	GCGTGAATAT GGTCTCCACC	Tsubota et al. (1999)	Bryopytes
rbcL	Reverse	rbcL804hR	TGCAGTAAAA CCACCTG	Tsubota et al. (1999)	Bryopytes
rbcL	Forward	rbcL650Rmas	CGATCTCTCC AACGCA	Masuzaki et al. (2010)	Bryopytes
rbcL	Reverse	rbcL600R	GTGAAATCAA GTCCACCACG	Tsubota et al. (1999)	Bryopytes
rbcL	Forward	rbcL302Rmas	ACATAAGCAA TATATTGATT	Masuzaki et al. (2010)	Bryopytes
rbcL	Reverse	rbcL270R	GCAATATATT GATTTTCTTC TCCAG	Tsubota et al. (1999)	Bryopytes
rbcL	Reverse	rbcL199R	GGTAAGTCCA TCAGTCCAAA C	Tsubota et al. (1999)	Bryopytes
rbcL	Forward	rbcL128Rmas	GTCATACGAA ATGCTGC	Masuzaki et al. (2010)	Bryopytes

• The PCR primer "rbcL-26Fmas" of Table 1 in Masuzaki et al. (2010a) was published with the sequences based on mistranscription of the sequences data, which should be rectified as "5'-TATTAATTGA TTTGTAGGGA GG-3'". In avoiding future confusion we gave a new name to the sequences (rbcL-26Fh: 5'-TATTAATTGA TTTGTAGGGA GG-3').

References

• Masuzaki, H., Shimamura, M., Furuki, T., Tsubota, H., Yamaguchi, T., A. M., Haji Mohamed & Deguchi, H. 2010. Systematic position of the enigmatic liverwort Mizutania (Mizutaniaceae, Marchantiophyta) inferred from molecular phylogenetic analyses. Taxon 59(2): 448-458.
• Tsubota, H., N. Nakao, T. Arikawa, T. Yamaguchi, M. Higuchi, H. Deguchi & T. Seki. 1999. A preliminary phylogeny of Hypnales (Musci) as inferred from chloroplast rbcL sequence data. Bryol. Res. 7: 233-248. pdf
• Tsubota, H., N. Nakao, T. Yamaguchi, T. Seki & H. Deguchi. 2000. Preliminary phylogenetic relationships of the genus Brotherella and its allied genera (Hypnales, Musci) based on chloroplast rbcL sequence data. J. Hattori Bot. Lab. 88: 79-99. Abstract
• Tsubota, H., H. Akiyama, T. Yamaguchi & H. Deguchi. 2001. Molecular phylogeny of the Sematophyllaceae (Hypnales, Musci) based on chloroplast rbcL sequences. J. Hattori Bot. Lab. 90: 221-240.

---

デジタル自然史博物館 / 広島大学 / 植物 / コケ植物（蘚苔類） / 情報 にもどる