「PCR primers cp rbcL」の版間の差分

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40行目: 40行目:
 
| ACAATGATAC TGTTTGTTAT AG
 
| ACAATGATAC TGTTTGTTAT AG
 
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
−
| Bryopytes
+
| Bryopytes, Vascular plants (''Sisymbrium'' etc.)
 
|-
 
|-
 
| rbcL
 
| rbcL
68行目: 68行目:
 
| ATGTCACCAC AAACGGAGAC TAAAGCAGG
 
| ATGTCACCAC AAACGGAGAC TAAAGCAGG
 
| [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]]
 
| [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]]
−
| Bryopytes
+
| Bryopytes, Vascular plants (''Sisymbrium'' etc.)
 
|-
 
|-
 
| rbcL
 
| rbcL
75行目: 75行目:
 
| TGGATTTAAA GCTGGTGTTA AAG
 
| TGGATTTAAA GCTGGTGTTA AAG
 
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
−
| Bryopytes
+
| Bryopytes, Vascular plants (''Sisymbrium'' etc.)
 
|-
 
|-
 
| rbcL
 
| rbcL
138行目: 138行目:
 
| TGTCTTCGTG GTGGAC
 
| TGTCTTCGTG GTGGAC
 
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
−
| Bryopytes
+
| Bryopytes, Vascular plants (''Sisymbrium'' etc.)
 
|-
 
|-
 
| rbcL
 
| rbcL
152行目: 152行目:
 
| CAATGCATGC AGTTATTGAC
 
| CAATGCATGC AGTTATTGAC
 
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
−
| Bryopytes
+
| Bryopytes, Vascular plants (''Sisymbrium'' etc.)
 
|-
 
|-
 
| rbcL
 
| rbcL
173行目: 173行目:
 
| GGTATGCATT TCCGTGTATT AGC
 
| GGTATGCATT TCCGTGTATT AGC
 
| Tsubota et al. (2001)
 
| Tsubota et al. (2001)
−
| Bryopytes
+
| Bryopytes, Vascular plants (''Sisymbrium'' etc.)
 
|-
 
|-
 
| rbcL
 
| rbcL
187行目: 187行目:
 
| GGTATTCATG TTTGGC
 
| GGTATTCATG TTTGGC
 
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
−
| Bryopytes
+
| Bryopytes, Vascular plants (''Sisymbrium'' etc.)
 
|-
 
|-
 
| rbcL
 
| rbcL
215行目: 215行目:
 
| ATGATATCCA CATTCTTCAC A
 
| ATGATATCCA CATTCTTCAC A
 
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
−
| Bryopytes
+
| Bryopytes, Vascular plants (''Sisymbrium'' etc.)
 
|-
 
|-
 
| rbcL
 
| rbcL
222行目: 222行目:
 
| GGGTTAGAAG GGATTCGAAC CCTTGAC
 
| GGGTTAGAAG GGATTCGAAC CCTTGAC
 
| Manhart (1994); [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| Manhart (1994); [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
−
| Bryopytes
+
| Bryopytes, Vascular plants (''Sisymbrium'' etc.)
 
|-
 
|-
 
| rbcL
 
| rbcL
250行目: 250行目:
 
| CTTCATTACG TGCTTGTACA CAAGCTTCTA
 
| CTTCATTACG TGCTTGTACA CAAGCTTCTA
 
| [[Inoue_et_al_2011|Inoue et al. (2011)]]
 
| [[Inoue_et_al_2011|Inoue et al. (2011)]]
−
| Bryopytes
+
| Bryopytes, Vascular plants (''Sisymbrium'' etc.)
 
|-
 
|-
 
| rbcL
 
| rbcL
257行目: 257行目:
 
| TTAATGCTGG CATATGCCAA AC
 
| TTAATGCTGG CATATGCCAA AC
 
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
−
| Bryopytes
+
| Bryopytes, Vascular plants (''Sisymbrium'' etc.)
 
|-
 
|-
 
| rbcL
 
| rbcL
299行目: 299行目:
 
| TGCAGTAAAA CCACCTG
 
| TGCAGTAAAA CCACCTG
 
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
−
| Bryopytes
+
| Bryopytes, Vascular plants (''Sisymbrium'' etc.)
 
|-
 
|-
 
| rbcL
 
| rbcL
306行目: 306行目:
 
| CGATCTCTCC AACGCA
 
| CGATCTCTCC AACGCA
 
| [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]]
 
| [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]]
−
| Bryopytes
+
| Bryopytes, Vascular plants (''Sisymbrium'' etc.)
 
|-
 
|-
 
| rbcL
 
| rbcL
313行目: 313行目:
 
| GTGAAATCAA GTCCACCACG
 
| GTGAAATCAA GTCCACCACG
 
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
 
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]]
−
| Bryopytes
+
| Bryopytes, Vascular plants (''Sisymbrium'' etc.)
 
|-
 
|-
 
| rbcL
 
| rbcL
327行目: 327行目:
 
| ATATCATAGC ATCGACCTTT GTAACGATC
 
| ATATCATAGC ATCGACCTTT GTAACGATC
 
| [[Inoue_et_al_2011|Inoue et al. (2011)]]
 
| [[Inoue_et_al_2011|Inoue et al. (2011)]]
−
| Bryopytes
+
| Bryopytes, Vascular plants (''Sisymbrium'' etc.)
 
|-
 
|-
 
| rbcL
 
| rbcL

2013年12月31日 (火) 19:56時点における版

広島大学 > デジタル自然史博物館 > 植物 > コケ植物（蘚苔類） > 情報

PCR primers

chloroplast

rbcL (atpB-rbcL-trnR-zfpA)

Table. Sequences (5’–3’) of synthetic primers used for PCR amplification and sequencing of the rbcL gene. The primer numbers from 5’ end correspond to rough positions in the sequence of Marchantia polymorpha (Ohyama et al. 1986).
Analyzed region Forward / Reverse Primer name Sequence (5'–3') References For
rbcL Forward atpB1011 GATCTACTAC TTTAATTCCT GT Tsubota et al. (1999) Bryopytes
rbcL Forward atpB370 AGTTGAATCT AAAGGATCTA C Tsubota et al. (1999) Bryopytes
rbcL Forward atbB175R TGTTGAACTT CACAAGTAAC A Manhart (1994) Bryopytes
rbcL Forward rbcL−130 ACAATGATAC TGTTTGTTAT AG Tsubota et al. (1999) Bryopytes, Vascular plants (Sisymbrium etc.)
rbcL Forward rbcL−73 ATACCAAAGA TGTTTTTTTA TAAG Tsubota et al. (1999) Bryopytes
rbcL Forward rbcL-26Fmas[1] AAGTCCCTCC CTACGAATCAA Masuzaki et al. (2010) Bryopytes
rbcL Forward rbcL-26Fh[1] TATTAATTGA TTTGTAGGGA GG Masuzaki et al. (2011) Bryopytes
rbcL Forward HrL1 ATGTCACCAC AAACGGAGAC TAAAGCAGG Masuzaki et al. (2010) Bryopytes, Vascular plants (Sisymbrium etc.)
rbcL Forward rbcL7 TGGATTTAAA GCTGGTGTTA AAG Tsubota et al. (1999) Bryopytes, Vascular plants (Sisymbrium etc.)
rbcL Forward rbcL39Fmas AAAGCTGGTG TTAAAGATTA Masuzaki et al. (2010) Bryopytes
rbcL Forward rbcL111Fmas GCAGCATTTC GTATGAC Masuzaki et al. (2010) Bryopytes
rbcL Forward rbcL152 GAATCCTCCA CTGGTACATG Tsubota et al. (1999) Bryopytes
rbcL Forward rbcL218h GACGATGCTA TGATCTCGAA GC Tsubota et al. (2000) Bryopytes
rbcL Forward rbcL256 GCTATGATCT TGAAGCAGTT CCTGGAGAAG Tsubota et al. (2000) Bryopytes
rbcL Forward rbcL265 ATTGCTTATG TTGCTTATCC Tsubota et al. (1999) Bryopytes
rbcL Forward rbcL333mas AATCAATATA TTGCTTATGT Masuzaki et al. (2011) Bryopytes
rbcL Forward rbcL557 GGTAGAGCTG TATATGAATG TCTTCGTGGT GG Tsubota et al. (2000) Bryopytes
rbcL Forward rbcL549 TGTCTTCGTG GTGGAC Tsubota et al. (1999) Bryopytes, Vascular plants (Sisymbrium etc.)
rbcL Forward rbcL634Fmas ATGCGTTGGA GAGA Masuzaki et al. (2010) Bryopytes
rbcL Forward rbcL862 CAATGCATGC AGTTATTGAC Tsubota et al. (1999) Bryopytes, Vascular plants (Sisymbrium etc.)
rbcL Forward rbcL919G CATGGTATGC ATTTCCGTGT A Tsubota et al. (2001) Bryopytes
rbcL Forward rbcL920Fmas CATGGTATGC ATTTCCGTGT Masuzaki et al. (2010) Bryopytes (Liverworts)
rbcL Forward rbcL921G GGTATGCATT TCCGTGTATT AGC Tsubota et al. (2001) Bryopytes, Vascular plants (Sisymbrium etc.)
rbcL Forward rbcL968G GAGATCATAT TCACGCTGGT AC Tsubota et al. (2001) Bryopytes
rbcL Forward rbcL1117 GGTATTCATG TTTGGC Tsubota et al. (1999) Bryopytes, Vascular plants (Sisymbrium etc.)
rbcL Forward rbcL1220 GGAGCAGTTG CTAATAGAG Tsubota et al. (1999) Bryopytes
rbcL Forward rbcL1276 GGACGTGATC TTGCTCG Tsubota et al. (1999) Bryopytes
rbcL Reverse zfpA546R ATGATATCCA CATTCTTCAC A Tsubota et al. (1999) Bryopytes, Vascular plants (Sisymbrium etc.)
rbcL Reverse trnRn GGGTTAGAAG GGATTCGAAC CCTTGAC Manhart (1994); Tsubota et al. (1999) Bryopytes, Vascular plants (Sisymbrium etc.)
rbcL Reverse trnR66R GAAGGGATTC GAACCCTTG Tsubota et al. (1999) Bryopytes
rbcL Reverse trnR24R CTCTAATCCA CTGAGCTACA Tsubota et al. (1999) Bryopytes
rbcL Reverse rbcL1346hR GCAGCTAATT CAGGACTCC Tsubota et al. (1999) Bryopytes
rbcL Reverse rbcL1301RL CTTCATTACG TGCTTGTACA CAAGCTTCTA Inoue et al. (2011) Bryopytes, Vascular plants (Sisymbrium etc.)
rbcL Reverse rbcL1145R TTAATGCTGG CATATGCCAA AC Tsubota et al. (1999) Bryopytes, Vascular plants (Sisymbrium etc.)
rbcL Reverse rbcL1098R AACACCTGGT AAAGAAACC Tsubota et al. (1999) Bryopytes
rbcL Reverse ArbcL-MR TGACGTTCTC CTTCAAGTTT ACCTACTAC Murakami et al. (1999) Ferns, Bryopytes
rbcL Reverse rbcL989R CTACGGTACC AGCGTGAATA TGGTCTCCAC CGG Tsubota et al. (2000) Bryopytes
rbcL Reverse rbcL957R GCGTGAATAT GGTCTCCACC Tsubota et al. (1999) Bryopytes
rbcL Forward HrL911R[2] Masuzaki et al. (2011) Bryopytes
rbcL Reverse rbcL804hR TGCAGTAAAA CCACCTG Tsubota et al. (1999) Bryopytes, Vascular plants (Sisymbrium etc.)
rbcL Forward rbcL650Rmas CGATCTCTCC AACGCA Masuzaki et al. (2010) Bryopytes, Vascular plants (Sisymbrium etc.)
rbcL Reverse rbcL600R[3] GTGAAATCAA GTCCACCACG Tsubota et al. (1999) Bryopytes, Vascular plants (Sisymbrium etc.)
rbcL Forward rbcL302Rmas ACATAAGCAA TATATTGATT Masuzaki et al. (2010) Bryopytes
rbcL Reverse HrL260R[4] ATATCATAGC ATCGACCTTT GTAACGATC Inoue et al. (2011) Bryopytes, Vascular plants (Sisymbrium etc.)
rbcL Reverse rbcL270R GCAATATATT GATTTTCTTC TCCAG Tsubota et al. (1999) Bryopytes
rbcL Reverse rbcL199R GGTAAGTCCA TCAGTCCAAA C Tsubota et al. (1999) Bryopytes
rbcL Forward rbcL128Rmas GTCATACGAA ATGCTGC Masuzaki et al. (2010) Bryopytes

脚注

  1. ↑ 1.0 1.1 The PCR primer "rbcL-26Fmas" of Table 1 in Masuzaki et al. (2010a) was published with the sequences based on mistranscription of the sequences data, which should be rectified as "5'-TATTAATTGA TTTGTAGGGA GG-3'". In avoiding future confusion we gave a new name to the sequences (rbcL-26Fh: 5'-TATTAATTGA TTTGTAGGGA GG-3').
  2. ↑ Masuzaki et al. (2011)のHrL911Rの配列は5'-CGTGCAATGC ATGCAGTTAT TGATAGACA-3'となっているが，誤り．
  3. ↑ Masuzaki et al. (2011)のrbcL600Rの配列は5'-CGTGGTGGAC TTGATTTCAC-3'となっているが，誤り．
  4. ↑ Inoue et al. (2011)では，Masuzaki et al. (2010)が初出となっているが，実際にはInoue et al. (2011)が初出．

References

  • Masuzaki, H., Shimamura, M., Furuki, T., Tsubota, H., Yamaguchi, T., A. M., Haji Mohamed & Deguchi, H. 2010. Systematic position of the enigmatic liverwort Mizutania (Mizutaniaceae, Marchantiophyta) inferred from molecular phylogenetic analyses. Taxon 59(2): 448-458.
  • Tsubota, H., N. Nakao, T. Arikawa, T. Yamaguchi, M. Higuchi, H. Deguchi & T. Seki. 1999. A preliminary phylogeny of Hypnales (Musci) as inferred from chloroplast rbcL sequence data. Bryol. Res. 7: 233-248. pdf
  • Tsubota, H., N. Nakao, T. Yamaguchi, T. Seki & H. Deguchi. 2000. Preliminary phylogenetic relationships of the genus Brotherella and its allied genera (Hypnales, Musci) based on chloroplast rbcL sequence data. J. Hattori Bot. Lab. 88: 79-99. Abstract
  • Tsubota, H., H. Akiyama, T. Yamaguchi & H. Deguchi. 2001. Molecular phylogeny of the Sematophyllaceae (Hypnales, Musci) based on chloroplast rbcL sequences. J. Hattori Bot. Lab. 90: 221-240.

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