「PCR primers cp rbcL」の版間の差分
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= [[PCR_primers|PCR primers]] = | = [[PCR_primers|PCR primers]] = | ||
== chloroplast == | == chloroplast == | ||
− | === rbcL (atpB-rbcL-trnR-zfpA) === | + | === ''rbcL'' (''atpB''-''rbcL''-''trnR''-''zfpA'') === |
{| class="wikitable" style="width:90%; margin:0 2em 0 2em; background-color:#d1d1a5; text-align:left" | {| class="wikitable" style="width:90%; margin:0 2em 0 2em; background-color:#d1d1a5; text-align:left" | ||
|+ style="text-align:left" | Table. Sequences (5’–3’) of synthetic primers used for PCR amplification and sequencing of the rbcL gene. The primer numbers from 5’ end correspond to rough positions in the sequence of ''Marchantia polymorpha'' (Ohyama et al. 1986). | |+ style="text-align:left" | Table. Sequences (5’–3’) of synthetic primers used for PCR amplification and sequencing of the rbcL gene. The primer numbers from 5’ end correspond to rough positions in the sequence of ''Marchantia polymorpha'' (Ohyama et al. 1986). |
2014年1月7日 (火) 14:38時点における版
広島大学 > デジタル自然史博物館 > 植物 > コケ植物(蘚苔類) > 情報
PCR primers
chloroplast
rbcL (atpB-rbcL-trnR-zfpA)
Analyzed region | Forward / Reverse | Primer name | Sequence (5'–3') | References | For |
rbcL | Forward | atpB1011 | GATCTACTAC TTTAATTCCT GT | Tsubota et al. (1999) | Bryopytes |
rbcL | Forward | atpB370 | AGTTGAATCT AAAGGATCTA C | Tsubota et al. (1999) | Bryopytes |
rbcL | Forward | atbB175R | TGTTGAACTT CACAAGTAAC A | Manhart (1994) | Bryopytes |
rbcL | Forward | rbcL−130 | ACAATGATAC TGTTTGTTAT AG | Tsubota et al. (1999) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Forward | rbcL−73 | ATACCAAAGA TGTTTTTTTA TAAG | Tsubota et al. (1999) | Bryopytes |
rbcL | Forward | rbcL-26Fmas[1] | AAGTCCCTCC CTACGAATCAA | Masuzaki et al. (2010) | Bryopytes |
rbcL | Forward | rbcL-26Fh[1] | TATTAATTGA TTTGTAGGGA GG | Masuzaki et al. (2011) | Bryopytes |
rbcL | Forward | HrL1 | ATGTCACCAC AAACGGAGAC TAAAGCAGG | Masuzaki et al. (2010) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Forward | rbcL7 | TGGATTTAAA GCTGGTGTTA AAG | Tsubota et al. (1999) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Forward | rbcL39Fmas | AAAGCTGGTG TTAAAGATTA | Masuzaki et al. (2010) | Bryopytes |
rbcL | Forward | rbcL111Fmas | GCAGCATTTC GTATGAC | Masuzaki et al. (2010) | Bryopytes |
rbcL | Forward | rbcL152 | GAATCCTCCA CTGGTACATG | Tsubota et al. (1999) | Bryopytes |
rbcL | Forward | rbcL218h | GACGATGCTA TGATCTCGAA GC | Tsubota et al. (2000) | Bryopytes |
rbcL | Forward | rbcL256 | GCTATGATCT TGAAGCAGTT CCTGGAGAAG | Tsubota et al. (2000) | Bryopytes |
rbcL | Forward | rbcL265 | ATTGCTTATG TTGCTTATCC | Tsubota et al. (1999) | Bryopytes |
rbcL | Forward | rbcL333mas | AATCAATATA TTGCTTATGT | Masuzaki et al. (2011) | Bryopytes |
rbcL | Forward | rbcL557 | GGTAGAGCTG TATATGAATG TCTTCGTGGT GG | Tsubota et al. (2000) | Bryopytes |
rbcL | Forward | rbcL549 | TGTCTTCGTG GTGGAC | Tsubota et al. (1999) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Forward | rbcL634Fmas | ATGCGTTGGA GAGA | Masuzaki et al. (2010) | Bryopytes |
rbcL | Forward | rbcL862 | CAATGCATGC AGTTATTGAC | Tsubota et al. (1999) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Forward | rbcL919G | CATGGTATGC ATTTCCGTGT A | Tsubota et al. (2001) | Bryopytes |
rbcL | Forward | rbcL920Fmas | CATGGTATGC ATTTCCGTGT | Masuzaki et al. (2010) | Bryopytes (Liverworts) |
rbcL | Forward | rbcL921G | GGTATGCATT TCCGTGTATT AGC | Tsubota et al. (2001) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Forward | rbcL968G | GAGATCATAT TCACGCTGGT AC | Tsubota et al. (2001) | Bryopytes |
rbcL | Forward | rbcL1117 | GGTATTCATG TTTGGC | Tsubota et al. (1999) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Forward | rbcL1220 | GGAGCAGTTG CTAATAGAG | Tsubota et al. (1999) | Bryopytes |
rbcL | Forward | rbcL1276 | GGACGTGATC TTGCTCG | Tsubota et al. (1999) | Bryopytes |
rbcL | Reverse | zfpA546R | ATGATATCCA CATTCTTCAC A | Tsubota et al. (1999) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Reverse | trnRn | GGGTTAGAAG GGATTCGAAC CCTTGAC | Manhart (1994); Tsubota et al. (1999) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Reverse | trnR66R | GAAGGGATTC GAACCCTTG | Tsubota et al. (1999) | Bryopytes |
rbcL | Reverse | trnR24R | CTCTAATCCA CTGAGCTACA | Tsubota et al. (1999) | Bryopytes |
rbcL | Reverse | rbcL1346hR | GCAGCTAATT CAGGACTCC | Tsubota et al. (1999) | Bryopytes |
rbcL | Reverse | rbcL1301RL | CTTCATTACG TGCTTGTACA CAAGCTTCTA | Inoue et al. (2011) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Reverse | rbcL1145R | TTAATGCTGG CATATGCCAA AC | Tsubota et al. (1999) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Reverse | rbcL1098R | AACACCTGGT AAAGAAACC | Tsubota et al. (1999) | Bryopytes |
rbcL | Reverse | ArbcL-MR | TGACGTTCTC CTTCAAGTTT ACCTACTAC | Murakami et al. (1999) | Ferns, Bryopytes |
rbcL | Reverse | rbcL989R | CTACGGTACC AGCGTGAATA TGGTCTCCAC CGG | Tsubota et al. (2000) | Bryopytes |
rbcL | Reverse | rbcL957R | GCGTGAATAT GGTCTCCACC | Tsubota et al. (1999) | Bryopytes |
rbcL | Forward | HrL911R[2] | Masuzaki et al. (2011) | Bryopytes | |
rbcL | Reverse | rbcL804hR | TGCAGTAAAA CCACCTG | Tsubota et al. (1999) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Forward | rbcL650Rmas | CGATCTCTCC AACGCA | Masuzaki et al. (2010) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Reverse | rbcL600R[3] | GTGAAATCAA GTCCACCACG | Tsubota et al. (1999) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Forward | rbcL302Rmas | ACATAAGCAA TATATTGATT | Masuzaki et al. (2010) | Bryopytes |
rbcL | Reverse | HrL260R[4][5] | ATATCATAGC ATCGACCTTT GTAACGATC | Inoue et al. (2011) | Bryopytes, Vascular plants (Sisymbrium etc.) |
rbcL | Reverse | rbcL270R | GCAATATATT GATTTTCTTC TCCAG | Tsubota et al. (1999) | Bryopytes |
rbcL | Reverse | rbcL199R | GGTAAGTCCA TCAGTCCAAA C | Tsubota et al. (1999) | Bryopytes |
rbcL | Forward | rbcL128Rmas | GTCATACGAA ATGCTGC | Masuzaki et al. (2010) | Bryopytes |
脚注
- ↑ 1.0 1.1 The PCR primer "rbcL-26Fmas" of Table 1 in Masuzaki et al. (2010a) was published with the sequences based on mistranscription of the sequences data, which should be rectified as "5'-TATTAATTGA TTTGTAGGGA GG-3'". In avoiding future confusion we gave a new name to the sequences (rbcL-26Fh: 5'-TATTAATTGA TTTGTAGGGA GG-3').
- ↑ Masuzaki et al. (2011)のHrL911Rの配列は5'-CGTGCAATGC ATGCAGTTAT TGATAGACA-3'となっているが,誤り.
- ↑ Masuzaki et al. (2011)のrbcL600Rの配列は5'-CGTGGTGGAC TTGATTTCAC-3'となっているが,誤り.
- ↑ Inoue et al. (2011)では,Masuzaki et al. (2010)が初出となっているが,実際にはInoue et al. (2011)が初出.
- ↑ The first appearance of this primer was in Table 1 of Inoue et al. (2011), and the footnote in Inoue et al. (2011) should be requested to make the corrections as the following: correct: “(published here)” and incorrect: “published by Masuzaki et al. (2010)”.
References
- Masuzaki, H., Shimamura, M., Furuki, T., Tsubota, H., Yamaguchi, T., A. M., Haji Mohamed & Deguchi, H. 2010. Systematic position of the enigmatic liverwort Mizutania (Mizutaniaceae, Marchantiophyta) inferred from molecular phylogenetic analyses. Taxon 59(2): 448-458.
- Tsubota, H., N. Nakao, T. Arikawa, T. Yamaguchi, M. Higuchi, H. Deguchi & T. Seki. 1999. A preliminary phylogeny of Hypnales (Musci) as inferred from chloroplast rbcL sequence data. Bryol. Res. 7: 233-248. pdf
- Tsubota, H., N. Nakao, T. Yamaguchi, T. Seki & H. Deguchi. 2000. Preliminary phylogenetic relationships of the genus Brotherella and its allied genera (Hypnales, Musci) based on chloroplast rbcL sequence data. J. Hattori Bot. Lab. 88: 79-99. Abstract
- Tsubota, H., H. Akiyama, T. Yamaguchi & H. Deguchi. 2001. Molecular phylogeny of the Sematophyllaceae (Hypnales, Musci) based on chloroplast rbcL sequences. J. Hattori Bot. Lab. 90: 221-240.
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