PCR primers

chloroplast

Table. Primers used for PCR amplification and sequencing
Analyzed region	Forward / Reverse	Primer name	Sequence (5'–3')	References	For
rbcL	Forward	atpB1011	GATCTACTAC TTTAATTCCT GT	Tsubota et al. (1999)	Bryopytes
rbcL	Forward	atpB370	AGTTGAATCT AAAGGATCTA C	Tsubota et al. (1999)	Bryopytes
rbcL	Forward	atbB175R	TGTTGAACTT CACAAGTAAC A	Manhart (1994)	Bryopytes
rbcL	Forward	rbcL−130	ACAATGATAC TGTTTGTTAT AG	Tsubota et al. (1999)	Bryopytes
rbcL	Forward	rbcL−73	ATACCAAAGA TGTTTTTTTA TAAG	Tsubota et al. (1999)	Bryopytes
rbcL	Forward	rbcL7	TGGATTTAAA GCTGGTGTTA AAG	Tsubota et al. (1999)	Bryopytes
rbcL	Forward	HrL1	ATGTCACCAC AAACGGAGAC TAAAGCAGG	Masuzaki et al. (2010)	Bryopytes
rbcL	Forward	rbcL256	GCTATGATCT TGAAGCAGTT CCTGGAGAAG	Tsubota et al. (2000)	Bryopytes
rbcL	Forward	rbcL557	GGTAGAGCTG TATATGAATG TCTTCGTGGT GG	Tsubota et al. (2000)	Bryopytes
rbcL	Forward	rbcL919G	CATGGTATGC ATTTCCGTGT A	Tsubota et al. (2001)	Bryopytes

rbcL	Reverse	trnRn	GGGTTAGAAG GGATTCGAAC CCTTGAC	Tsubota et al. (1999)	Bryopytes
rbcL	Reverse	rbcL1346hR	GCAGCTAATT CAGGACTCC	Tsubota et al. (1999)	Bryopytes
rbcL	Reverse	rbcL1301RL	CTTCATTACG TGCTTGTACA CAAGCTTCTA	Inoue et al. (2011)	Bryopytes
rbcL	Reverse	rbcL1098R	AACACCTGGT AAAGAAACC	Tsubota et al. (1999)	Bryopytes
rbcL	Reverse	rbcL600R	GTGAAATCAA GTCCACCACG	Tsubota et al. (1999)	Bryopytes

Handa, S., Nakahara, M., Tsubota, H., Deguchi, H. & Nakano, T. 2003. A new aerial alga, Stichococcus ampulliformis sp. nov. (Trebouxiophyceae, Chlorophyta) from Japan. Phycol. Res. 51: 203-210.
Taberlet, P., Gielly, L., Pautou, G. & Bouvet, J. 1991. Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol. Biol. 17: 1105-1109.
Tsubota, H., Kubo, H. & Mukai, S. 2009. A new finding of seagrass Halophila nipponica J.Kuo (Hydrocharitaceae) from Miyajima Island in Hiroshima Prefecture, SW Japan. Hikobia 15: 339-347 (in Japanese with English Abstract and Tables). [坪田博美・久保晴盛・向井誠二. 2009. 広島県宮島近海で見つかったヤマトウミヒルモHalophila nipponica J.Kuoについて. Hikobia 15: 339-347.]
Tsubota, H., Kubo, H., Takeuchi, K., Nakahara-Tsubota, M., Inoue, Y., Uchida, S. & Mukai, S. 2012. Notes on naturalized plants in Hiroshima Prefecture: 3. New locality and nrITS variation of Lactuca serriola L. (Asteraceae). Hikobia 16: 197-202 (in Japanese with English Abstract and Tables). [坪田博美・久保晴盛・武内一恵・中原-坪田美保・井上侑哉・内田慎治・向井誠二. 2012. 広島の帰化植物3.トゲヂシャとマルバトゲヂシャ. Hikobia 16: 197-202.]