「PCR primers cp rps4-trnT-trnL-trnF」の版間の差分

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=[[PCR_primers|PCR primers]]=
 
=[[PCR_primers|PCR primers]]=
 
==chloroplast==
 
==chloroplast==
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===''rps4''===
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===''rps4'' (ribosomal protein S4; ribosomal protein subunit 4)===
 
{| class="wikitable" style="width:90%; margin:0 2em 0 2em; background-color:#d1d1a5; text-align:left"
 
{| class="wikitable" style="width:90%; margin:0 2em 0 2em; background-color:#d1d1a5; text-align:left"
 
|+ style="text-align:left" | Table. Primers used for PCR amplification and sequencing
 
|+ style="text-align:left" | Table. Primers used for PCR amplification and sequencing
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| References
 
| References
 
| For
 
| For
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|-
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|''rps4''
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| Forward
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| trnT36R
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| GTAATGCGAT GGTCATCGGT TCGACTCCGA TA
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| [[Inoue_et_al_2012|Inoue et al. (2012)]]
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| Bryophytes
 
|-
 
|-
 
|''rps4''
 
|''rps4''
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|}
 
|}
  
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==[[テンプレート:PCR_primers_References|References]]==
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=== ''rps4''–''trnT''–''trnL''–''trnF'' ===
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{| class="wikitable" style="width:90%; margin:0 2em 0 2em; background-color:#d1d1a5; text-align:left"
 +
|+ style="text-align:left" | Table. Primers used for PCR amplification and sequencing
 +
|-
 +
| Analyzed region
 +
| Forward / Reverse
 +
| Primer name
 +
| Sequence (5'–3')
 +
| References
 +
| For
 +
|-
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| ''rps4''–''trnT''–''trnL''–''trnF''
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| Forward
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| rps4_609RL
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| TTAAGCTTGA CGAGAATAAT ATTC
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| [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]]
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| Bryophytes ([[Masuzaki_et_al_2010|Masuzaki et al. 2010]]); Vascular plants (''Lysimachia'', [[Tsubota_et_al_2015b|Tsubota et al. 2015b]])
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|-
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| ''trnT''–''trnL''–''trnF''
 +
| Forward
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| trnT71F
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| CCGCTATCGG AGTCGAACCG ATGACCATCG
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| [[坪田・池田・井上・内田・向井_2015|Tsubota et al. (2015b)]]
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| Vascular plants (''Lysimachia'', [[Tsubota_et_al_2015b|Tsubota et al. 2015b]])
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|-
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| ''trnL''–''trnF''
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| Forward
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| a [trnT(UGU)F-a]
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| CATTACAAAT GCGATGCTCT
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| [[Taberlet_Gielly_Pautou_Bouvet_1991|Taberlet et al. (1991)]]
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| Vascular plants
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|-
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| ''trnL''–''trnF''
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| Forward
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| c [trnL(UAA)5exonF-c]
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| CGAAATCGGT AGACGCTACG
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| [[Taberlet_Gielly_Pautou_Bouvet_1991|Taberlet et al. (1991)]]
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| Vascular plants
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|-
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| ''trnL''–''trnF''
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| Forward
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| g
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| GGGCAATCCT GAGCCAA
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| [[Taberlet_Coissac_Pompanon_Gielly_Miquel_Valentini_Vermat_Corthier_Brochmann_Willerslev_2007|Taberlet et al. (2007)]]
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| Vascular plants
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|-
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| ''trnL''–''trnF''
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| Forward
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| e [trnL(UAA)3exonF-e]
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| GGTTCAAGTC CCTCTATCCC
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| [[Taberlet_Gielly_Pautou_Bouvet_1991|Taberlet et al. (1991)]]
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| Vascular plants
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|-
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|
 +
|
 +
|
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|
 +
|
 +
|
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|-
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| ''rps4''–''trnT''–''trnL''–''trnF''
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| Reverse
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| trnF1F
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| TGCCAGAAAC CAGATTTGAA CTGGTGACAC
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| [[坪田・池田・井上・内田・向井_2015|Tsubota et al. (2015b)]]
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| Vascular plants (''Lysimachia'', [[Tsubota_et_al_2015b|Tsubota et al. 2015b]])
 +
|-
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| ''rps4''–''trnT''–''trnL''–''trnF''
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| Reverse
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| trnF8F
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| TCAGTCCTCT GCTCTACCAA CTGAGCTA
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| [[坪田・池田・井上・内田・向井_2015|Tsubota et al. (2015b)]]
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| Vascular plants (''Lysimachia'', [[Tsubota_et_al_2015b|Tsubota et al. 2015b]])
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|-
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| ''trnL''–''trnF''
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| Reverse
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| f [trnF(GAA)R-f]
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| ATTTGAACTG GTGACACGAG
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| [[Taberlet_Gielly_Pautou_Bouvet_1991|Taberlet et al. (1991)]]
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| Vascular plants
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|-
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| ''trnL''–''trnF''
 +
| Reverse
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| d [trnL(UAA)3exonR-d]
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| GGGGATAGAG GGACTTGAAC
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| [[Taberlet_Gielly_Pautou_Bouvet_1991|Taberlet et al. (1991)]]
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| Vascular plants
 +
|-
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| ''trnL''–''trnF''
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| Reverse
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| h
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| CCATTGAGTC TCTGCACCTA TC
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| [[Taberlet_Coissac_Pompanon_Gielly_Miquel_Valentini_Vermat_Corthier_Brochmann_Willerslev_2007|Taberlet et al. (2007)]]
 +
| Vascular plants
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|-
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| ''trnL''–''trnF''
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| Reverse
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| b [trnL(UAA)5exonR-b]
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| TCTACCGATT TCGCCATATC
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| [[Taberlet_Gielly_Pautou_Bouvet_1991|Taberlet et al. (1991)]]
 +
| Vascular plants
 +
|}
 +
 
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==Example of primer pairs==
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*trnF1F & trnS (for ''rps4''-''trnT''-''trnL''-''trnF'')
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*trnF1F & trnT71F (for ''trnL''-''trnF'' region)
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*trnT36R & trnS (for ''rps4'' gene)
 +
 
 +
=[[テンプレート:PCR_primers_References|References]]=
 
{{PCR_primers_References}}
 
{{PCR_primers_References}}
 +
 +
==[[テンプレート:primers|関連ページ]]==
 +
{{primers}}
  
 
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[http://www.digital-museum.hiroshima-u.ac.jp/~museum/ デジタル自然史博物館] / [http://www.hiroshima-u.ac.jp/index-j.html 広島大学] / [http://www.digital-museum.hiroshima-u.ac.jp/~museum/plant/ 植物] / [[コケ|コケ植物（蘚苔類）]] / [[コケ/情報|情報]] にもどる
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[https://www.digital-museum.hiroshima-u.ac.jp/~museum/ デジタル自然史博物館] / [https://www.hiroshima-u.ac.jp 広島大学] / [https://www.digital-museum.hiroshima-u.ac.jp/~museum/plant/ 植物] / [[コケ|コケ植物（蘚苔類）]] / [[コケ/情報|情報]] にもどる
  
 
[[Category:広島大学デジタル自然史博物館]]
 
[[Category:広島大学デジタル自然史博物館]]

2023年12月4日 (月) 16:43時点における最新版

広島大学 > デジタル自然史博物館 > 植物 > コケ植物（蘚苔類） > 情報

PCR primers

chloroplast

rps4 (ribosomal protein S4; ribosomal protein subunit 4)

Table. Primers used for PCR amplification and sequencing
Analyzed region Forward / Reverse Primer name Sequence (5'–3') References For
rps4 Forward trnT36R GTAATGCGAT GGTCATCGGT TCGACTCCGA TA Inoue et al. (2012) Bryophytes
rps4 Forward rps5 ATGTCCCGTT ATCGAGGACC T Nadot et al. (1994) Bryophytes
rps4 Forward rps4_1R ATGTCCCGTT ATCGAGGACC TCGTGTA Inoue et al. (2012) Bryophytes
rps4 Forward rps4_2Fmas TGTCCCGTTA TCGAGGACC Masuzaki et al. (2010) Bryophytes
rps4 Forward rps4_19Fi CCTCGTGTAA GAATAATACG TC Inoue & Tsubota (2014) Bryophytes
rps4 Forward rps4_275Fmas AGATAATGTG ATCTTTCGAT TAGGTATGGC Masuzaki et al. (2010) Bryophytes
rps4 Forward rps4_347Fmas CAATTGGTTA ATCATAGACA Masuzaki et al. (2010) Bryophytes
rps4 Forward rps4_553Fmas CAAACGATTA ATCGTGAATG GA Masuzaki et al. (2010) Bryophytes
rps4 Reverse psaA340F CTTGAGCACT AGGTTTAATA TGAGTAGGAT CA Inoue et al. (2012) Bryophytes
rps4 Reverse trnS (syn. trans) TACCGAGGGT TCGAATC Souza-Chies et al. (1997), Buck et al. (2000) Vascular plants (Souza-Chies et al. 1997), Bryophytes (Buck et al. 2000, as trans)
rps4 Reverse rps4_609RL TTAAGCTTGA CGAGAATAAT ATTC Masuzaki et al. (2010) Bryophytes
rps4 Reverse psa3' ATATTCTACA ACTAACAACT C Nadot et al. (1994) Bryophytes
rps4 Reverse rps4_602Fn (psa4_602Fn) TGACGAGAAT AATATTCTAC AACTA Inoue & Tsubota (2014) Bryophytes
rps4 Reverse rps4_605Rmas ACTTGGCGAG AATAATATTC AAC Masuzaki et al. (2010) Bryophytes
rps4 Reverse rps4_578Ri CGAGAATAAT ATTCTACAAC TA Inoue & Tsubota (2014) Bryophytes
rps4 Reverse rps4_531Rmas TCCATTCACG ATTAATCGTT TG Masuzaki et al. (2010) Bryophytes
rps4 Reverse rps4_305Rmas GCCATACCTA ATCGAAAGAT CACATTATCT Masuzaki et al. (2010) Bryophytes

rps4–trnT–trnL–trnF

Table. Primers used for PCR amplification and sequencing
Analyzed region Forward / Reverse Primer name Sequence (5'–3') References For
rps4–trnT–trnL–trnF Forward rps4_609RL TTAAGCTTGA CGAGAATAAT ATTC Masuzaki et al. (2010) Bryophytes (Masuzaki et al. 2010); Vascular plants (Lysimachia, Tsubota et al. 2015b)
trnT–trnL–trnF Forward trnT71F CCGCTATCGG AGTCGAACCG ATGACCATCG Tsubota et al. (2015b) Vascular plants (Lysimachia, Tsubota et al. 2015b)
trnL–trnF Forward a [trnT(UGU)F-a] CATTACAAAT GCGATGCTCT Taberlet et al. (1991) Vascular plants
trnL–trnF Forward c [trnL(UAA)5exonF-c] CGAAATCGGT AGACGCTACG Taberlet et al. (1991) Vascular plants
trnL–trnF Forward g GGGCAATCCT GAGCCAA Taberlet et al. (2007) Vascular plants
trnL–trnF Forward e [trnL(UAA)3exonF-e] GGTTCAAGTC CCTCTATCCC Taberlet et al. (1991) Vascular plants
rps4–trnT–trnL–trnF Reverse trnF1F TGCCAGAAAC CAGATTTGAA CTGGTGACAC Tsubota et al. (2015b) Vascular plants (Lysimachia, Tsubota et al. 2015b)
rps4–trnT–trnL–trnF Reverse trnF8F TCAGTCCTCT GCTCTACCAA CTGAGCTA Tsubota et al. (2015b) Vascular plants (Lysimachia, Tsubota et al. 2015b)
trnL–trnF Reverse f [trnF(GAA)R-f] ATTTGAACTG GTGACACGAG Taberlet et al. (1991) Vascular plants
trnL–trnF Reverse d [trnL(UAA)3exonR-d] GGGGATAGAG GGACTTGAAC Taberlet et al. (1991) Vascular plants
trnL–trnF Reverse h CCATTGAGTC TCTGCACCTA TC Taberlet et al. (2007) Vascular plants
trnL–trnF Reverse b [trnL(UAA)5exonR-b] TCTACCGATT TCGCCATATC Taberlet et al. (1991) Vascular plants

Example of primer pairs

  • trnF1F & trnS (for rps4-trnT-trnL-trnF)
  • trnF1F & trnT71F (for trnL-trnF region)
  • trnT36R & trnS (for rps4 gene)

References

関連ページ

デジタル自然史博物館 / 広島大学 / 植物 / コケ植物（蘚苔類） / 情報 にもどる

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