「PCR primers」の版間の差分
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| GGTTCAAGTC CCTCTATCCC | | GGTTCAAGTC CCTCTATCCC | ||
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2015年12月31日 (木) 02:12時点における版
広島大学 > デジタル自然史博物館 > 植物 > コケ植物（蘚苔類） > 情報
PCR primers
chloroplast
nuclear
PCR primers
chloroplast
rbcL (atpB-rbcL-trnR-zfpA)
rps4
| Analyzed region | Forward / Reverse | Primer name | Sequence (5'–3') | References | For |
| rps4 | Forward | rps5 | ATGTCCCGTT ATCGAGGACC T | Nadot et al. (1994) | Bryophytes |
| rps4 | Forward | rps4_1R | ATGTCCCGTT ATCGAGGACC TCGTGTA | Inoue et al. (2012) | Bryophytes |
| rps4 | Forward | rps4_2Fmas | TGTCCCGTTA TCGAGGACC | Masuzaki et al. (2010) | Bryophytes |
| rps4 | Forward | rps4_19Fi | CCTCGTGTAA GAATAATACG TC | Inoue & Tsubota (2014) | Bryophytes |
| rps4 | Forward | rps4_275Fmas | AGATAATGTG ATCTTTCGAT TAGGTATGGC | Masuzaki et al. (2010) | Bryophytes |
| rps4 | Forward | rps4_347Fmas | CAATTGGTTA ATCATAGACA | Masuzaki et al. (2010) | Bryophytes |
| rps4 | Forward | rps4_553Fmas | CAAACGATTA ATCGTGAATG GA | Masuzaki et al. (2010) | Bryophytes |
| rps4 | Reverse | psaA340F | CTTGAGCACT AGGTTTAATA TGAGTAGGAT CA | Inoue et al. (2012) | Bryophytes |
| rps4 | Reverse | trnS (syn. trans) | TACCGAGGGT TCGAATC | Souza-Chies et al. (1997), Buck et al. (2000) | Vascular plants (Souza-Chies et al. 1997), Bryophytes (Buck et al. 2000, as trans) |
| rps4 | Reverse | rps4_609RL | TTAAGCTTGA CGAGAATAAT ATTC | Masuzaki et al. (2010) | Bryophytes |
| rps4 | Reverse | psa3' | ATATTCTACA ACTAACAACT C | Nadot et al. (1994) | Bryophytes |
| rps4 | Reverse | rps4_602Fn ( |
TGACGAGAAT AATATTCTAC AACTA | Inoue & Tsubota (2014) | Bryophytes |
| rps4 | Reverse | rps4_605Rmas | ACTTGGCGAG AATAATATTC AAC | Masuzaki et al. (2010) | Bryophytes |
| rps4 | Reverse | rps4_578Ri | CGAGAATAAT ATTCTACAAC TA | Inoue & Tsubota (2014) | Bryophytes |
| rps4 | Reverse | rps4_531Rmas | TCCATTCACG ATTAATCGTT TG | Masuzaki et al. (2010) | Bryophytes |
| rps4 | Reverse | rps4_305Rmas | GCCATACCTA ATCGAAAGAT CACATTATCT | Masuzaki et al. (2010) | Bryophytes |
matK
| Analyzed region | Forward / Reverse | Primer name | Sequence (5'–3') | References | For |
| trnK intron (including matK) | trnK [tRNA-Lys(UAA)exon1] | CCGACTAGTT CCGGGTTCGA | Demesure et al. (1995) | ||
| trnK aF | ARWTTCATCC AAACCATTGA CAAGG | Oguri et al. (2013) | |||
| matK 410F | TATCAATCTA TTCATTCYGT ATTTCCTTTT | Oguri et al. (2013) | |||
| matK 410R | AAAAGGAAAT ACRGAATGAA TAGATTGATA | Oguri et al. (2013) | |||
| trnK aR | ATTGCACACG GCTTTCTCTA TGT | Oguri et al. (2013) | |||
| trnK [tRNA-Lys(UAA)exon2] | CAACGGTAGA GTACTCGGCT TTTA | Demesure et al. (1995) |
trnL-F
| Analyzed region | Forward / Reverse | Primer name | Sequence (5'–3') | References | For |
| trnL-F | Forward | a [trnT(UGU)F-a] | CATTACAAAT GCGATGCTCT | Taberlet et al. (1991) | |
| trnL-F | Forward | c [trnL(UAA)5exonF-c] | CGAAATCGGT AGACGCTACG | Taberlet et al. (1991) | |
| trnL-F | Forward | e [trnL(UAA)3exonF-e] | GGTTCAAGTC CCTCTATCCC | Taberlet et al. (1991) | |
| trnL-F | Reverse | f [trnF(GAA)R-f] | ATTTGAACTG GTGACACGAG | Taberlet et al. (1991) | |
| trnL-F | Reverse | d [trnL(UAA)3exonR-d] | GGGGATAGAG GGACTTGAAC | Taberlet et al. (1991) | |
| trnL-F | Reverse | b [trnL(UAA)5exonR-b] | TCTACCGATT TCGCCATATC | Taberlet et al. (1991) |
psbA-trnH [trnH(GUG)-psbA intergenic region, trnH/psbA spacer]
| Analyzed region | Forward / Reverse | Primer name | Sequence (5'–3') | References | For |
| psbA-trnH | Forward | psbAF | GTTATGCATG AACGTAATGC TC | Sang et al. (1997) | Vascular plants (Sang et al. 1997) |
| psbA-trnH | Forward (as Reverse in Hamilton (1999)) | psb_A | CGAAGCTCCA TCTACAAATG G | Hamilton (1999) | Vascular plants (Hamilton 1999) |
| psbA-trnH | Reverse (as Forward in Hamilton (1999)) | trn_H_(GUG) | ACTGCCTTGA TCCACTTGGC | Hamilton (1999) | Vascular plants (Hamilton 1999) |
| psbA-trnH | Reverse | trnHR | CGCGCATGGT GGATTCACAA ATC | Sang et al. (1997) | Vascular plants (Sang et al. 1997) |
nuclear
18S rRNA gene and ITS region
References
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デジタル自然史博物館 / 広島大学 / 植物 / コケ植物（蘚苔類） / 情報 にもどる
