「PCR primers」の版間の差分

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===''psbA''-''trnH'' or ''trnH''-''psbA'' [''trnH''(GUG)-''psbA'' intergenic region; ''trnH''/''psbA'' spacer; chloroplast photosystem II protein D1 (''psbA'') gene, partial cds; ''psbA''-''trnH'' intergenic spacer, complete sequence; and tRNA-His (''trnH'') gene, partial sequence] ===
 
===''psbA''-''trnH'' or ''trnH''-''psbA'' [''trnH''(GUG)-''psbA'' intergenic region; ''trnH''/''psbA'' spacer; chloroplast photosystem II protein D1 (''psbA'') gene, partial cds; ''psbA''-''trnH'' intergenic spacer, complete sequence; and tRNA-His (''trnH'') gene, partial sequence] ===
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{| class="wikitable" style="width:90%; margin:0 2em 0 2em; background-color:#d1d1a5; text-align:left"
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|+ style="text-align:left" | Table. Primers used for PCR amplification and sequencing
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|-
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| Analyzed region
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| style="width: 150px"| Forward / Reverse
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| style="width: 200px"| Primer name
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| style="width: 200px"| Sequence (5'–3')
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| References
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| For
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|-
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| ''psbA''-''trnH''
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| Forward
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| psbAFh5s
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| GTTATGCATG AACGTAA
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| [[坪田・惠良・池田・久保・武内・中原-坪田・内田・向井_2015|Tsubota et al. (2015a)]]
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| Vascular plants (''Cotula'', [[坪田・惠良・池田・久保・武内・中原-坪田・内田・向井_2015|Tsubota et al. 2015a]])
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|-
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| ''psbA''-''trnH''
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| Forward
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| psbAF (as Reverse primer named psbA-R in [[Newmaster_Ragupathy_2009|Newmaster & Ragupathy 2009]])
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| GTTATGCATG AACGTAATGC TC
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| [[Sang_Crawford_Stuessy_1997|Sang et al. (1997)]], [[Newmaster_Ragupathy_2009|Newmaster & Ragupathy (2009)]]
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| Vascular plants ([[Sang_Crawford_Stuessy_1997|Sang et al. 1997]], [[Newmaster_Ragupathy_2009|Fabaceae by Newmaster & Ragupathy 2009]], [[Bruni_Galimberti_Caridi_Scaccabarozzi_DeMattia_Casiraghi_Labra_2015|multiflower honey by Bruni et al. 2015]])
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|-
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| ''psbA''-''trnH''
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| Forward (as Reverse in [[Hamilton_1999|Hamilton 1999]])
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| psb_A
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| CGAAGCTCCA TCTACAAATG G
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| [[Hamilton_1999|Hamilton (1999)]]
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| Vascular plants ([[Hamilton_1999|Hamilton 1999]])
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|-
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|
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|
 +
|
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|
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|
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|
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|-
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| ''psbA''-''trnH''
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| Reverse
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| trnHRh6s
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| CGCGCATGGT GGATTCA
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| [[坪田・惠良・池田・久保・武内・中原-坪田・内田・向井_2015|Tsubota et al. (2015a)]]
 +
| Vascular plants (''Cotula'', [[坪田・惠良・池田・久保・武内・中原-坪田・内田・向井_2015|Tsubota et al. 2015a]])
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|-
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| ''psbA''-''trnH''
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| Reverse
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| trnHR
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| CGCGCATGGT GGATTCACAA ATC
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| [[Sang_Crawford_Stuessy_1997|Sang et al. (1997)]]
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| Vascular plants ([[Sang_Crawford_Stuessy_1997|Sang et al. 1997]])
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|-
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| ''psbA''-''trnH''
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| Reverse
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| trnHf_05 (as Forward primer named trnH-F in [[Newmaster_Ragupathy_2009|Newmaster & Ragupathy 2009]])
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| CGCGCATGGT GGATTCACAA TCC
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| [[Tate_Simpson_2003|Tate & Simpson 2003]], [[Newmaster_Ragupathy_2009|Newmaster & Ragupathy (2009)]]
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| Vascular plants ([[Tate_Simpson_2003|''Tarasa ''by Tate & Simpson 2003]], [[Newmaster_Ragupathy_2009|Fabaceae by Newmaster & Ragupathy 2009]], [[Bruni_Galimberti_Caridi_Scaccabarozzi_DeMattia_Casiraghi_Labra_2015|multiflower honey by Bruni et al. 2015]])
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|-
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| ''psbA''-''trnH''
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| Reverse (as Forward in [[Hamilton_1999|Hamilton 1999]])
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| trn_H_(GUG)
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| ACTGCCTTGA TCCACTTGGC
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| [[Hamilton_1999|Hamilton (1999)]]
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| Vascular plants ([[Hamilton_1999|Hamilton 1999]])
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|}
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 +
===''rpl16 G2 intron'' [''rpl16 G2 intron'' region] ===
 
{| class="wikitable" style="width:90%; margin:0 2em 0 2em; background-color:#d1d1a5; text-align:left"
 
{| class="wikitable" style="width:90%; margin:0 2em 0 2em; background-color:#d1d1a5; text-align:left"
 
|+ style="text-align:left" | Table. Primers used for PCR amplification and sequencing
 
|+ style="text-align:left" | Table. Primers used for PCR amplification and sequencing

2018年4月11日 (水) 13:56時点における版

広島大学 > デジタル自然史博物館 > 植物 > コケ植物（蘚苔類） > 情報

PCR primers

chloroplast

nuclear

PCR primers

chloroplast

rbcL (atpB-rbcL-trnR-zfpA)

rps4

rps4–trnT–trnL–trnF

Table. Primers used for PCR amplification and sequencing
Analyzed region Forward / Reverse Primer name Sequence (5'–3') References For
rps4–trnT–trnL–trnF Forward rps4_609RL TTAAGCTTGA CGAGAATAAT ATTC Masuzaki et al. (2010) Bryophytes (Masuzaki et al. 2010); Vascular plants (Lysimachia, Tsubota et al. 2015b)
trnT–trnL–trnF Forward trnT71F CCGCTATCGG AGTCGAACCG ATGACCATCG Tsubota et al. (2015b) Vascular plants (Lysimachia, Tsubota et al. 2015b)
trnL–trnF Forward a [trnT(UGU)F-a] CATTACAAAT GCGATGCTCT Taberlet et al. (1991) Vascular plants
trnL–trnF Forward c [trnL(UAA)5exonF-c] CGAAATCGGT AGACGCTACG Taberlet et al. (1991) Vascular plants
trnL–trnF Forward e [trnL(UAA)3exonF-e] GGTTCAAGTC CCTCTATCCC Taberlet et al. (1991) Vascular plants
rps4–trnT–trnL–trnF Reverse trnF1F TGCCAGAAAC CAGATTTGAA CTGGTGACAC Tsubota et al. (2015b) Vascular plants (Lysimachia, Tsubota et al. 2015b)
rps4–trnT–trnL–trnF Reverse trnF8F TCAGTCCTCT GCTCTACCAA CTGAGCTA Tsubota et al. (2015b) Vascular plants (Lysimachia, Tsubota et al. 2015b)
trnL–trnF Reverse f [trnF(GAA)R-f] ATTTGAACTG GTGACACGAG Taberlet et al. (1991) Vascular plants
trnL–trnF Reverse d [trnL(UAA)3exonR-d] GGGGATAGAG GGACTTGAAC Taberlet et al. (1991) Vascular plants
trnL–trnF Reverse b [trnL(UAA)5exonR-b] TCTACCGATT TCGCCATATC Taberlet et al. (1991) Vascular plants

matK

Table. Primers used for PCR amplification and sequencing
Analyzed region Forward / Reverse Primer name Sequence (5'–3') References For
trnK intron (including matK) trnK [tRNA-Lys(UAA)exon1] CCGACTAGTT CCGGGTTCGA Demesure et al. (1995) Bryophytes (Leucobryum, Oguri et al. 2013)
trnK aF ARWTTCATCC AAACCATTGA CAAGG Oguri et al. (2013) Bryophytes (Leucobryum, Oguri et al. 2013)
matK 410F TATCAATCTA TTCATTCYGT ATTTCCTTTT Oguri et al. (2013) Bryophytes (Leucobryum, Oguri et al. 2013)
matK 410R AAAAGGAAAT ACRGAATGAA TAGATTGATA Oguri et al. (2013) Bryophytes (Leucobryum, Oguri et al. 2013)
trnK aR ATTGCACACG GCTTTCTCTA TGT Oguri et al. (2013) Bryophytes (Leucobryum, Oguri et al. 2013)
trnK [tRNA-Lys(UAA)exon2] CAACGGTAGA GTACTCGGCT TTTA Demesure et al. (1995) Bryophytes (Leucobryum, Oguri et al. 2013)

psbA-trnH or trnH-psbA [trnH(GUG)-psbA intergenic region; trnH/psbA spacer; chloroplast photosystem II protein D1 (psbA) gene, partial cds; psbA-trnH intergenic spacer, complete sequence; and tRNA-His (trnH) gene, partial sequence]

Table. Primers used for PCR amplification and sequencing
Analyzed region Forward / Reverse Primer name Sequence (5'–3') References For
psbA-trnH Forward psbAFh5s GTTATGCATG AACGTAA Tsubota et al. (2015a) Vascular plants (Cotula, Tsubota et al. 2015a)
psbA-trnH Forward psbAF (as Reverse primer named psbA-R in Newmaster & Ragupathy 2009) GTTATGCATG AACGTAATGC TC Sang et al. (1997), Newmaster & Ragupathy (2009) Vascular plants (Sang et al. 1997, Fabaceae by Newmaster & Ragupathy 2009, multiflower honey by Bruni et al. 2015)
psbA-trnH Forward (as Reverse in Hamilton 1999) psb_A CGAAGCTCCA TCTACAAATG G Hamilton (1999) Vascular plants (Hamilton 1999)
psbA-trnH Reverse trnHRh6s CGCGCATGGT GGATTCA Tsubota et al. (2015a) Vascular plants (Cotula, Tsubota et al. 2015a)
psbA-trnH Reverse trnHR CGCGCATGGT GGATTCACAA ATC Sang et al. (1997) Vascular plants (Sang et al. 1997)
psbA-trnH Reverse trnHf_05 (as Forward primer named trnH-F in Newmaster & Ragupathy 2009) CGCGCATGGT GGATTCACAA TCC Tate & Simpson 2003, Newmaster & Ragupathy (2009) Vascular plants (Tarasa by Tate & Simpson 2003, Fabaceae by Newmaster & Ragupathy 2009, multiflower honey by Bruni et al. 2015)
psbA-trnH Reverse (as Forward in Hamilton 1999) trn_H_(GUG) ACTGCCTTGA TCCACTTGGC Hamilton (1999) Vascular plants (Hamilton 1999)

rpl16 G2 intron [rpl16 G2 intron region]

Table. Primers used for PCR amplification and sequencing
Analyzed region Forward / Reverse Primer name Sequence (5'–3') References For
psbA-trnH Forward psbAFh5s GTTATGCATG AACGTAA Tsubota et al. (2015a) Vascular plants (Cotula, Tsubota et al. 2015a)
psbA-trnH Forward psbAF (as Reverse primer named psbA-R in Newmaster & Ragupathy 2009) GTTATGCATG AACGTAATGC TC Sang et al. (1997), Newmaster & Ragupathy (2009) Vascular plants (Sang et al. 1997, Fabaceae by Newmaster & Ragupathy 2009, multiflower honey by Bruni et al. 2015)
psbA-trnH Forward (as Reverse in Hamilton 1999) psb_A CGAAGCTCCA TCTACAAATG G Hamilton (1999) Vascular plants (Hamilton 1999)
psbA-trnH Reverse trnHRh6s CGCGCATGGT GGATTCA Tsubota et al. (2015a) Vascular plants (Cotula, Tsubota et al. 2015a)
psbA-trnH Reverse trnHR CGCGCATGGT GGATTCACAA ATC Sang et al. (1997) Vascular plants (Sang et al. 1997)
psbA-trnH Reverse trnHf_05 (as Forward primer named trnH-F in Newmaster & Ragupathy 2009) CGCGCATGGT GGATTCACAA TCC Tate & Simpson 2003, Newmaster & Ragupathy (2009) Vascular plants (Tarasa by Tate & Simpson 2003, Fabaceae by Newmaster & Ragupathy 2009, multiflower honey by Bruni et al. 2015)
psbA-trnH Reverse (as Forward in Hamilton 1999) trn_H_(GUG) ACTGCCTTGA TCCACTTGGC Hamilton (1999) Vascular plants (Hamilton 1999)

nuclear

18S rRNA gene and ITS region

References

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