「PCR primers cp rbcL」の版間の差分
提供: 広島大学デジタル博物館
ナビゲーションに移動検索に移動| (2人の利用者による、間の17版が非表示) | |||
| 19行目: | 19行目: | ||
| GATCTACTAC TTTAATTCCT GT | | GATCTACTAC TTTAATTCCT GT | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''atpB''-''rbcL'' | | ''atpB''-''rbcL'' | ||
| 26行目: | 26行目: | ||
| AGTTGAATCT AAAGGATCTA C | | AGTTGAATCT AAAGGATCTA C | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''atpB''-''rbcL'' | | ''atpB''-''rbcL'' | ||
| 33行目: | 33行目: | ||
| TGTTGAACTT CACAAGTAAC A | | TGTTGAACTT CACAAGTAAC A | ||
| [[Manhart_1994|Manhart (1994)]] | | [[Manhart_1994|Manhart (1994)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 40行目: | 40行目: | ||
| ACAATGATAC TGTTTGTTAT AG | | ACAATGATAC TGTTTGTTAT AG | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes, Vascular plants (''Sisymbrium'' etc.) |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 47行目: | 47行目: | ||
| ATACCAAAGA TGTTTTTTTA TAAG | | ATACCAAAGA TGTTTTTTTA TAAG | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 54行目: | 54行目: | ||
| TCGAGTAGAC CTTATCCTTG C | | TCGAGTAGAC CTTATCCTTG C | ||
| [[Inoue_Tsubota_2014|Inoue & Tsubota (2014)]] | | [[Inoue_Tsubota_2014|Inoue & Tsubota (2014)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 61行目: | 61行目: | ||
| AAGTCCCTCC CTACGAATCAA | | AAGTCCCTCC CTACGAATCAA | ||
| [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | | [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 68行目: | 68行目: | ||
| TATTAATTGA TTTGTAGGGA GG | | TATTAATTGA TTTGTAGGGA GG | ||
| [[Masuzaki_et_al_2011|Masuzaki et al. (2011)]] | | [[Masuzaki_et_al_2011|Masuzaki et al. (2011)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 75行目: | 75行目: | ||
| ATGTCACCAC AAACGGAGAC TAAAGCAGG | | ATGTCACCAC AAACGGAGAC TAAAGCAGG | ||
| [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | | [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | ||
| − | | | + | | Bryophytes (''Frullania'' etc.), Vascular plants (''Sisymbrium'', ''Acer'' etc.) |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 82行目: | 82行目: | ||
| TGGATTTAAA GCTGGTGTTA AAG | | TGGATTTAAA GCTGGTGTTA AAG | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes (''Frullania'' etc.), Vascular plants (''Sisymbrium'', ''Acer'', ''Pinus'' etc.) |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 89行目: | 89行目: | ||
| AAAGCTGGTG TTAAAGATTA | | AAAGCTGGTG TTAAAGATTA | ||
| [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | | [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 96行目: | 96行目: | ||
| GCAGCATTTC GTATGAC | | GCAGCATTTC GTATGAC | ||
| [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | | [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 103行目: | 103行目: | ||
| GAATCCTCCA CTGGTACATG | | GAATCCTCCA CTGGTACATG | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 110行目: | 110行目: | ||
| GACGATGCTA TGATCTCGAA GC | | GACGATGCTA TGATCTCGAA GC | ||
| [[Tsubota_Nakao_Yamaguchi_Seki_Deguchi_2000|Tsubota et al. (2000)]] | | [[Tsubota_Nakao_Yamaguchi_Seki_Deguchi_2000|Tsubota et al. (2000)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 117行目: | 117行目: | ||
| GCTATGATCT TGAAGCAGTT CCTGGAGAAG | | GCTATGATCT TGAAGCAGTT CCTGGAGAAG | ||
| [[Tsubota_Nakao_Yamaguchi_Seki_Deguchi_2000|Tsubota et al. (2000)]] | | [[Tsubota_Nakao_Yamaguchi_Seki_Deguchi_2000|Tsubota et al. (2000)]] | ||
| − | | | + | | Bryophytes |
| + | |- | ||
| + | | ''rbcL'' | ||
| + | | Forward | ||
| + | | rbcL333mas | ||
| + | | AATCAATATA TTGCTTATGT | ||
| + | | [[Masuzaki_et_al_2011|Masuzaki et al. (2011)]] | ||
| + | | Bryophytes | ||
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 124行目: | 131行目: | ||
| ATTGCTTATG TTGCTTATCC | | ATTGCTTATG TTGCTTATCC | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes |
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 138行目: | 138行目: | ||
| GGTAGAGCTG TATATGAATG TCTTCGTGGT GG | | GGTAGAGCTG TATATGAATG TCTTCGTGGT GG | ||
| [[Tsubota_Nakao_Yamaguchi_Seki_Deguchi_2000|Tsubota et al. (2000)]] | | [[Tsubota_Nakao_Yamaguchi_Seki_Deguchi_2000|Tsubota et al. (2000)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 145行目: | 145行目: | ||
| TGTCTTCGTG GTGGAC | | TGTCTTCGTG GTGGAC | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes (''Frullania'' etc.), Vascular plants (''Sisymbrium'', ''Pinus'' etc.) |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 152行目: | 152行目: | ||
| ATGCGTTGGA GAGA | | ATGCGTTGGA GAGA | ||
| [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | | [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | ||
| − | | | + | | Bryophytes |
| + | |- | ||
| + | | ''rbcL'' | ||
| + | | Forward | ||
| + | | rbcL862h1s | ||
| + | | CAATGCATGC AGTTATTGA | ||
| + | | [[中村・本郷・松坂・長﨑・池田・塩路・清水・坪田_2022|Nakamura et al. (2022)]] | ||
| + | | Vascular plants (''Acer'' etc.) | ||
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 159行目: | 166行目: | ||
| CAATGCATGC AGTTATTGAC | | CAATGCATGC AGTTATTGAC | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes, Vascular plants (''Sisymbrium'', ''Pinus'' etc.) |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 166行目: | 173行目: | ||
| CATGGTATGC ATTTCCGTGT A | | CATGGTATGC ATTTCCGTGT A | ||
| [[Tsubota_Akiyama_Yamaguchi_Deguchi_2001|Tsubota et al. (2001)]] | | [[Tsubota_Akiyama_Yamaguchi_Deguchi_2001|Tsubota et al. (2001)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 173行目: | 180行目: | ||
| CATGGTATGC ATTTCCGTGT | | CATGGTATGC ATTTCCGTGT | ||
| [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | | [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | ||
| − | | | + | | Bryophytes (Liverworts (''Frullania'' etc.)), Vascular plants (''Pinus'' etc.) |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 180行目: | 187行目: | ||
| GGTATGCATT TCCGTGTATT AGC | | GGTATGCATT TCCGTGTATT AGC | ||
| [[Tsubota_Akiyama_Yamaguchi_Deguchi_2001|Tsubota et al. (2001)]] | | [[Tsubota_Akiyama_Yamaguchi_Deguchi_2001|Tsubota et al. (2001)]] | ||
| − | | | + | | Bryophytes, Vascular plants (''Sisymbrium'' etc.) |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 187行目: | 194行目: | ||
| GAGATCATAT TCACGCTGGT AC | | GAGATCATAT TCACGCTGGT AC | ||
| [[Tsubota_Akiyama_Yamaguchi_Deguchi_2001|Tsubota et al. (2001)]] | | [[Tsubota_Akiyama_Yamaguchi_Deguchi_2001|Tsubota et al. (2001)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 194行目: | 201行目: | ||
| GGTATTCATG TTTGGC | | GGTATTCATG TTTGGC | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes, Vascular plants (''Sisymbrium'' etc.) |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 201行目: | 208行目: | ||
| GGAGCAGTTG CTAATAGAG | | GGAGCAGTTG CTAATAGAG | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 208行目: | 215行目: | ||
| GGACGTGATC TTGCTCG | | GGACGTGATC TTGCTCG | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| | | | ||
| 222行目: | 229行目: | ||
| ATGATATCCA CATTCTTCAC A | | ATGATATCCA CATTCTTCAC A | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes, Vascular plants (''Sisymbrium'' etc.) |
|- | |- | ||
| ''rbcL''-''trnR'' | | ''rbcL''-''trnR'' | ||
| 229行目: | 236行目: | ||
| GGGTTAGAAG GGATTCGAAC CCTTGAC | | GGGTTAGAAG GGATTCGAAC CCTTGAC | ||
| [[Manhart_1994|Manhart (1994)]]; [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Manhart_1994|Manhart (1994)]]; [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes, Vascular plants (''Sisymbrium'' etc.) |
|- | |- | ||
| ''rbcL''-''trnR'' | | ''rbcL''-''trnR'' | ||
| 236行目: | 243行目: | ||
| GAAGGGATTC GAACCCTTG | | GAAGGGATTC GAACCCTTG | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''rbcL''-''trnR'' | | ''rbcL''-''trnR'' | ||
| 243行目: | 250行目: | ||
| CTCTAATCCA CTGAGCTACA | | CTCTAATCCA CTGAGCTACA | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 250行目: | 257行目: | ||
| GCAGCTAATT CAGGACTCC | | GCAGCTAATT CAGGACTCC | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes (''Frullania'' etc.) |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 257行目: | 264行目: | ||
| CTTCATTACG TGCTTGTACA CAAGCTTCTA | | CTTCATTACG TGCTTGTACA CAAGCTTCTA | ||
| [[Inoue_et_al_2011|Inoue et al. (2011)]] | | [[Inoue_et_al_2011|Inoue et al. (2011)]] | ||
| − | | | + | | Bryophytes (''Frullania'' etc.), Vascular plants (''Sisymbrium'', ''Acer'' etc.) |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 264行目: | 271行目: | ||
| TTAATGCTGG CATATGCCAA AC | | TTAATGCTGG CATATGCCAA AC | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes, Vascular plants (''Sisymbrium'' etc.) |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 271行目: | 278行目: | ||
| AACACCTGGT AAAGAAACC | | AACACCTGGT AAAGAAACC | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 278行目: | 285行目: | ||
| TGACGTTCTC CTTCAAGTTT ACCTACTAC | | TGACGTTCTC CTTCAAGTTT ACCTACTAC | ||
| Murakami et al. (1999) | | Murakami et al. (1999) | ||
| − | | Ferns, | + | | Ferns, Bryophytes |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 285行目: | 292行目: | ||
| CTACGGTACC AGCGTGAATA TGGTCTCCAC CGG | | CTACGGTACC AGCGTGAATA TGGTCTCCAC CGG | ||
| [[Tsubota_Nakao_Yamaguchi_Seki_Deguchi_2000|Tsubota et al. (2000)]] | | [[Tsubota_Nakao_Yamaguchi_Seki_Deguchi_2000|Tsubota et al. (2000)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 292行目: | 299行目: | ||
| GCGTGAATAT GGTCTCCACC | | GCGTGAATAT GGTCTCCACC | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 299行目: | 306行目: | ||
| CGTGCAATGC ATGCAGTTAT TGATAGACA | | CGTGCAATGC ATGCAGTTAT TGATAGACA | ||
| [[Masuzaki_et_al_2011|Masuzaki et al. (2011)]] | | [[Masuzaki_et_al_2011|Masuzaki et al. (2011)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| Reverse | | Reverse | ||
| − | | HrL911Rtm<ref name=" | + | | HrL911Rtm<ref name="chu6">Masuzaki et al.（2011）の配列を訂正</ref> |
| TGTCTATCAA TAACTGCATG CATTGCACG | | TGTCTATCAA TAACTGCATG CATTGCACG | ||
| [[Phan_Nakahara-Tsubota_Inoue_Tsubota_2021|Phan et al. (2021)]] | | [[Phan_Nakahara-Tsubota_Inoue_Tsubota_2021|Phan et al. (2021)]] | ||
| − | | | + | | Bryophytes, Vascular plants (''Dysphania'' etc.) |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 313行目: | 320行目: | ||
| TGCAGTAAAA CCACCTG | | TGCAGTAAAA CCACCTG | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes, Vascular plants (''Sisymbrium'' etc.) |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 320行目: | 327行目: | ||
| CGATCTCTCC AACGCA | | CGATCTCTCC AACGCA | ||
| [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | | [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | ||
| − | | | + | | Bryophytes (''Frullania'' etc.), Vascular plants (''Sisymbrium'', ''Acer'' etc.) |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 327行目: | 334行目: | ||
| GTGAAATCAA GTCCACCACG | | GTGAAATCAA GTCCACCACG | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes, Vascular plants (''Sisymbrium'', ''Pinus'' etc.) |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 334行目: | 341行目: | ||
| ACATAAGCAA TATATTGATT | | ACATAAGCAA TATATTGATT | ||
| [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | | [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 341行目: | 348行目: | ||
| ATATCATAGC ATCGACCTTT GTAACGATC | | ATATCATAGC ATCGACCTTT GTAACGATC | ||
| [[Inoue_et_al_2011|Inoue et al. (2011)]] | | [[Inoue_et_al_2011|Inoue et al. (2011)]] | ||
| − | | | + | | Bryophytes, Vascular plants (''Sisymbrium'' etc.) |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 348行目: | 355行目: | ||
| GCAATATATT GATTTTCTTC TCCAG | | GCAATATATT GATTTTCTTC TCCAG | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 355行目: | 362行目: | ||
| GGTAAGTCCA TCAGTCCAAA C | | GGTAAGTCCA TCAGTCCAAA C | ||
| [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | | [[Tsubota_Nakao_Arikawa_Yamaguchi_Higuchi_Deguchi_Seki_1999|Tsubota et al. (1999)]] | ||
| − | | | + | | Bryophytes |
|- | |- | ||
| ''rbcL'' | | ''rbcL'' | ||
| 362行目: | 369行目: | ||
| GTCATACGAA ATGCTGC | | GTCATACGAA ATGCTGC | ||
| [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | | [[Masuzaki_Shimamura_Furuki_Tsubota_Yamaguchi_Haji_Deguchi_2010|Masuzaki et al. (2010)]] | ||
| − | | | + | | Bryophytes |
| + | |} | ||
| + | |||
| + | === [[rbcL|cp ''atpB'' gene, ''atpB-rbcL'' region and ''rbcL'' gene]] === | ||
| + | {| class="wikitable" style="width:90%; margin:0 2em 0 2em; background-color:#d1d1a5; text-align:left" | ||
| + | |+ style="text-align:left" | Table. Sequences (5’–3’) of synthetic primers used for PCR amplification and sequencing of the ''atpB'' gene, ''atpB-rbcL'' region and ''rbcL'' gene. The primer numbers from 5’ end correspond to rough positions in the sequence of ''Marchantia polymorpha'' ([http://www.nature.com/nature/journal/v322/n6079/abs/322572a0.html Ohyama et al. 1986]). | ||
| + | |- | ||
| + | | Analyzed region | ||
| + | | Forward / Reverse | ||
| + | | Primer name | ||
| + | | Sequence (5'–3') | ||
| + | | References | ||
| + | | For | ||
| + | |- | ||
| + | | ''atpB-rbcL'' | ||
| + | | Forward | ||
| + | | PZatpB1334R | ||
| + | | <tt>GGAGAACCCG TAAATACTTC TGCTA</tt> | ||
| + | | [[中村・小山・ファン・中原-坪田・坪田_2023|Nakamura et al. (2023)]] | ||
| + | | Vascular plants (''Lonicera'' etc.) | ||
| + | |- | ||
| + | | ''atpB-rbcL'' | ||
| + | | Forward | ||
| + | | PZatpB1226R | ||
| + | | <tt>CCAAGAATAG CTATAATATC TTGA</tt> | ||
| + | | [[中村・小山・ファン・中原-坪田・坪田_2023|Nakamura et al. (2023)]] | ||
| + | | Vascular plants (''Lonicera'' etc.) | ||
| + | |- | ||
| + | | ''atpB-rbcL'' | ||
| + | | Forward | ||
| + | | PZatpB1157R | ||
| + | | <tt>TCATAATGTT CTTCACCAAC GA</tt> | ||
| + | | [[中村・小山・ファン・中原-坪田・坪田_2023|Nakamura et al. (2023)]] | ||
| + | | Vascular plants (''Lonicera'' etc.) | ||
| + | |- | ||
| + | | ''atpB-rbcL'' | ||
| + | | Forward | ||
| + | | PZatpB1061R | ||
| + | | <tt>CTTGATAGTA CGGTAGTA</tt> | ||
| + | | [[中村・小山・ファン・中原-坪田・坪田_2023|Nakamura et al. (2023)]] | ||
| + | | Vascular plants (''Lonicera'' etc.) | ||
| + | |- | ||
| + | | ''atpB-rbcL'' | ||
| + | | Forward | ||
| + | | PZatpB911R | ||
| + | | <tt>GTACTAAGGG TAGGTTGATA</tt> | ||
| + | | [[中村・小山・ファン・中原-坪田・坪田_2023|Nakamura et al. (2023)]] | ||
| + | | Vascular plants (''Lonicera'' etc.) | ||
| + | |- | ||
| + | | ''atpB-rbcL'' | ||
| + | | Forward | ||
| + | | PZatpB557R | ||
| + | | <tt>ATCAATTCCA TAATGAGTA</tt> | ||
| + | | [[中村・小山・ファン・中原-坪田・坪田_2023|Nakamura et al. (2023)]] | ||
| + | | Vascular plants (''Lonicera'' etc.) | ||
| + | |- | ||
| + | | ''atpB-rbcL'' | ||
| + | | Forward | ||
| + | | PZatpB212R | ||
| + | | <tt>TTTCCTAATA ATTGCTGTAC CTCACA</tt> | ||
| + | | [[中村・小山・ファン・中原-坪田・坪田_2023|Nakamura et al. (2023)]] | ||
| + | | Vascular plants (''Lonicera'' etc.) | ||
| + | |- | ||
| + | | | ||
| + | | | ||
| + | | | ||
| + | | | ||
| + | | | ||
| + | | | ||
| + | |- | ||
| + | | ''atpB-rbcL'' | ||
| + | | Reverse | ||
| + | | PrL728RL | ||
| + | | <tt>GTAGCATTCA AGTAATGCCC TTTGATTTCA</tt> | ||
| + | | [[中村・小山・ファン・中原-坪田・坪田_2023|Nakamura et al. (2023)]] | ||
| + | | Vascular plants (''Lonicera'' etc.) | ||
| + | |- | ||
| + | | ''atpB-rbcL'' | ||
| + | | Reverse | ||
| + | | Rbc60Lh3s | ||
| + | | <tt>CAGGAGTATA ATAAGTCA</tt> | ||
| + | | [[中村・小山・ファン・中原-坪田・坪田_2023|Nakamura et al. (2023)]] | ||
| + | | Vascular plants (''Lonicera'' etc.) | ||
| + | |- | ||
| + | | ''atpB-rbcL'' | ||
| + | | Reverse | ||
| + | | PZatpB184F | ||
| + | | <tt>ACTTGTGAGG TACAGCAATT ATTAGGA</tt> | ||
| + | | [[中村・小山・ファン・中原-坪田・坪田_2023|Nakamura et al. (2023)]] | ||
| + | | Vascular plants (''Lonicera'' etc.) | ||
| + | |- | ||
| + | | ''atpB-rbcL'' | ||
| + | | Reverse | ||
| + | | PZatpB538F | ||
| + | | <tt>GTACTCATTA TGGAATTGA</tt> | ||
| + | | [[中村・小山・ファン・中原-坪田・坪田_2023|Nakamura et al. (2023)]] | ||
| + | | Vascular plants (''Lonicera'' etc.) | ||
| + | |- | ||
| + | | ''atpB-rbcL'' | ||
| + | | Reverse | ||
| + | | PZatpB1042F | ||
| + | | <tt>GCTACTACCG TACTATCA</tt> | ||
| + | | [[中村・小山・ファン・中原-坪田・坪田_2023|Nakamura et al. (2023)]] | ||
| + | | Vascular plants (''Lonicera'' etc.) | ||
|} | |} | ||
| + | |||
| + | ==Example of primer pairs for PCR== | ||
| + | *rbcL-53h & trnR24R (for ''rbcL'' gene) | ||
| + | *HrL1 & rbcL1346hR (for ''rbcL'' gene) | ||
| + | *rbcL7 & rbcL1301RL (for ''rbcL'' gene) | ||
| + | *HrL1 & rbcL1301RL (for ''rbcL'' gene) | ||
| + | *PZatpB1334R-PrL728RL (for ''atpB-rbcL'') | ||
== 脚注 == | == 脚注 == | ||
2024年2月14日 (水) 21:56時点における最新版
広島大学 > デジタル自然史博物館 > 植物 > コケ植物（蘚苔類） > 情報
PCR primers
chloroplast
rbcL (atpB-rbcL-trnR-zfpA)
| Analyzed region | Forward / Reverse | Primer name | Sequence (5'–3') | References | For |
| atpB-rbcL | Forward | atpB1011 | GATCTACTAC TTTAATTCCT GT | Tsubota et al. (1999) | Bryophytes |
| atpB-rbcL | Forward | atpB370 | AGTTGAATCT AAAGGATCTA C | Tsubota et al. (1999) | Bryophytes |
| atpB-rbcL | Forward | atbB175R | TGTTGAACTT CACAAGTAAC A | Manhart (1994) | Bryophytes |
| rbcL | Forward | rbcL−130 | ACAATGATAC TGTTTGTTAT AG | Tsubota et al. (1999) | Bryophytes, Vascular plants (Sisymbrium etc.) |
| rbcL | Forward | rbcL−73 | ATACCAAAGA TGTTTTTTTA TAAG | Tsubota et al. (1999) | Bryophytes |
| rbcL | Forward | rbcL−53h | TCGAGTAGAC CTTATCCTTG C | Inoue & Tsubota (2014) | Bryophytes |
| rbcL | Forward | rbcL-26Fmas[1] | AAGTCCCTCC CTACGAATCAA | Masuzaki et al. (2010) | Bryophytes |
| rbcL | Forward | rbcL-26Fh[1] | TATTAATTGA TTTGTAGGGA GG | Masuzaki et al. (2011) | Bryophytes |
| rbcL | Forward | HrL1 | ATGTCACCAC AAACGGAGAC TAAAGCAGG | Masuzaki et al. (2010) | Bryophytes (Frullania etc.), Vascular plants (Sisymbrium, Acer etc.) |
| rbcL | Forward | rbcL7 | TGGATTTAAA GCTGGTGTTA AAG | Tsubota et al. (1999) | Bryophytes (Frullania etc.), Vascular plants (Sisymbrium, Acer, Pinus etc.) |
| rbcL | Forward | rbcL39Fmas | AAAGCTGGTG TTAAAGATTA | Masuzaki et al. (2010) | Bryophytes |
| rbcL | Forward | rbcL111Fmas | GCAGCATTTC GTATGAC | Masuzaki et al. (2010) | Bryophytes |
| rbcL | Forward | rbcL152 | GAATCCTCCA CTGGTACATG | Tsubota et al. (1999) | Bryophytes |
| rbcL | Forward | rbcL218h | GACGATGCTA TGATCTCGAA GC | Tsubota et al. (2000) | Bryophytes |
| rbcL | Forward | rbcL256 | GCTATGATCT TGAAGCAGTT CCTGGAGAAG | Tsubota et al. (2000) | Bryophytes |
| rbcL | Forward | rbcL333mas | AATCAATATA TTGCTTATGT | Masuzaki et al. (2011) | Bryophytes |
| rbcL | Forward | rbcL265 | ATTGCTTATG TTGCTTATCC | Tsubota et al. (1999) | Bryophytes |
| rbcL | Forward | rbcL557 | GGTAGAGCTG TATATGAATG TCTTCGTGGT GG | Tsubota et al. (2000) | Bryophytes |
| rbcL | Forward | rbcL549 | TGTCTTCGTG GTGGAC | Tsubota et al. (1999) | Bryophytes (Frullania etc.), Vascular plants (Sisymbrium, Pinus etc.) |
| rbcL | Forward | rbcL634Fmas | ATGCGTTGGA GAGA | Masuzaki et al. (2010) | Bryophytes |
| rbcL | Forward | rbcL862h1s | CAATGCATGC AGTTATTGA | Nakamura et al. (2022) | Vascular plants (Acer etc.) |
| rbcL | Forward | rbcL862 | CAATGCATGC AGTTATTGAC | Tsubota et al. (1999) | Bryophytes, Vascular plants (Sisymbrium, Pinus etc.) |
| rbcL | Forward | rbcL919G | CATGGTATGC ATTTCCGTGT A | Tsubota et al. (2001) | Bryophytes |
| rbcL | Forward | rbcL920Fmas | CATGGTATGC ATTTCCGTGT | Masuzaki et al. (2010) | Bryophytes (Liverworts (Frullania etc.)), Vascular plants (Pinus etc.) |
| rbcL | Forward | rbcL921G | GGTATGCATT TCCGTGTATT AGC | Tsubota et al. (2001) | Bryophytes, Vascular plants (Sisymbrium etc.) |
| rbcL | Forward | rbcL968G | GAGATCATAT TCACGCTGGT AC | Tsubota et al. (2001) | Bryophytes |
| rbcL | Forward | rbcL1117 | GGTATTCATG TTTGGC | Tsubota et al. (1999) | Bryophytes, Vascular plants (Sisymbrium etc.) |
| rbcL | Forward | rbcL1220 | GGAGCAGTTG CTAATAGAG | Tsubota et al. (1999) | Bryophytes |
| rbcL | Forward | rbcL1276 | GGACGTGATC TTGCTCG | Tsubota et al. (1999) | Bryophytes |
| rbcL-trnR-zfpA | Reverse | zfpA546R | ATGATATCCA CATTCTTCAC A | Tsubota et al. (1999) | Bryophytes, Vascular plants (Sisymbrium etc.) |
| rbcL-trnR | Reverse | trnRn | GGGTTAGAAG GGATTCGAAC CCTTGAC | Manhart (1994); Tsubota et al. (1999) | Bryophytes, Vascular plants (Sisymbrium etc.) |
| rbcL-trnR | Reverse | trnR66R | GAAGGGATTC GAACCCTTG | Tsubota et al. (1999) | Bryophytes |
| rbcL-trnR | Reverse | trnR24R | CTCTAATCCA CTGAGCTACA | Tsubota et al. (1999) | Bryophytes |
| rbcL | Reverse | rbcL1346hR | GCAGCTAATT CAGGACTCC | Tsubota et al. (1999) | Bryophytes (Frullania etc.) |
| rbcL | Reverse | rbcL1301RL | CTTCATTACG TGCTTGTACA CAAGCTTCTA | Inoue et al. (2011) | Bryophytes (Frullania etc.), Vascular plants (Sisymbrium, Acer etc.) |
| rbcL | Reverse | rbcL1145R | TTAATGCTGG CATATGCCAA AC | Tsubota et al. (1999) | Bryophytes, Vascular plants (Sisymbrium etc.) |
| rbcL | Reverse | rbcL1098R | AACACCTGGT AAAGAAACC | Tsubota et al. (1999) | Bryophytes |
| rbcL | Reverse | ArbcL-MR | TGACGTTCTC CTTCAAGTTT ACCTACTAC | Murakami et al. (1999) | Ferns, Bryophytes |
| rbcL | Reverse | rbcL989R | CTACGGTACC AGCGTGAATA TGGTCTCCAC CGG | Tsubota et al. (2000) | Bryophytes |
| rbcL | Reverse | rbcL957R | GCGTGAATAT GGTCTCCACC | Tsubota et al. (1999) | Bryophytes |
| rbcL | Reverse | HrL911R[2] | CGTGCAATGC ATGCAGTTAT TGATAGACA | Masuzaki et al. (2011) | Bryophytes |
| rbcL | Reverse | HrL911Rtm[3] | TGTCTATCAA TAACTGCATG CATTGCACG | Phan et al. (2021) | Bryophytes, Vascular plants (Dysphania etc.) |
| rbcL | Reverse | rbcL804hR | TGCAGTAAAA CCACCTG | Tsubota et al. (1999) | Bryophytes, Vascular plants (Sisymbrium etc.) |
| rbcL | Reverse | rbcL650Rmas | CGATCTCTCC AACGCA | Masuzaki et al. (2010) | Bryophytes (Frullania etc.), Vascular plants (Sisymbrium, Acer etc.) |
| rbcL | Reverse | rbcL600R[4] | GTGAAATCAA GTCCACCACG | Tsubota et al. (1999) | Bryophytes, Vascular plants (Sisymbrium, Pinus etc.) |
| rbcL | Reverse | rbcL302Rmas | ACATAAGCAA TATATTGATT | Masuzaki et al. (2010) | Bryophytes |
| rbcL | Reverse | HrL260R[5][6] | ATATCATAGC ATCGACCTTT GTAACGATC | Inoue et al. (2011) | Bryophytes, Vascular plants (Sisymbrium etc.) |
| rbcL | Reverse | rbcL270R | GCAATATATT GATTTTCTTC TCCAG | Tsubota et al. (1999) | Bryophytes |
| rbcL | Reverse | rbcL199R | GGTAAGTCCA TCAGTCCAAA C | Tsubota et al. (1999) | Bryophytes |
| rbcL | Reverse | rbcL128Rmas | GTCATACGAA ATGCTGC | Masuzaki et al. (2010) | Bryophytes |
cp atpB gene, atpB-rbcL region and rbcL gene
| Analyzed region | Forward / Reverse | Primer name | Sequence (5'–3') | References | For |
| atpB-rbcL | Forward | PZatpB1334R | GGAGAACCCG TAAATACTTC TGCTA | Nakamura et al. (2023) | Vascular plants (Lonicera etc.) |
| atpB-rbcL | Forward | PZatpB1226R | CCAAGAATAG CTATAATATC TTGA | Nakamura et al. (2023) | Vascular plants (Lonicera etc.) |
| atpB-rbcL | Forward | PZatpB1157R | TCATAATGTT CTTCACCAAC GA | Nakamura et al. (2023) | Vascular plants (Lonicera etc.) |
| atpB-rbcL | Forward | PZatpB1061R | CTTGATAGTA CGGTAGTA | Nakamura et al. (2023) | Vascular plants (Lonicera etc.) |
| atpB-rbcL | Forward | PZatpB911R | GTACTAAGGG TAGGTTGATA | Nakamura et al. (2023) | Vascular plants (Lonicera etc.) |
| atpB-rbcL | Forward | PZatpB557R | ATCAATTCCA TAATGAGTA | Nakamura et al. (2023) | Vascular plants (Lonicera etc.) |
| atpB-rbcL | Forward | PZatpB212R | TTTCCTAATA ATTGCTGTAC CTCACA | Nakamura et al. (2023) | Vascular plants (Lonicera etc.) |
| atpB-rbcL | Reverse | PrL728RL | GTAGCATTCA AGTAATGCCC TTTGATTTCA | Nakamura et al. (2023) | Vascular plants (Lonicera etc.) |
| atpB-rbcL | Reverse | Rbc60Lh3s | CAGGAGTATA ATAAGTCA | Nakamura et al. (2023) | Vascular plants (Lonicera etc.) |
| atpB-rbcL | Reverse | PZatpB184F | ACTTGTGAGG TACAGCAATT ATTAGGA | Nakamura et al. (2023) | Vascular plants (Lonicera etc.) |
| atpB-rbcL | Reverse | PZatpB538F | GTACTCATTA TGGAATTGA | Nakamura et al. (2023) | Vascular plants (Lonicera etc.) |
| atpB-rbcL | Reverse | PZatpB1042F | GCTACTACCG TACTATCA | Nakamura et al. (2023) | Vascular plants (Lonicera etc.) |
Example of primer pairs for PCR
- rbcL-53h & trnR24R (for rbcL gene)
- HrL1 & rbcL1346hR (for rbcL gene)
- rbcL7 & rbcL1301RL (for rbcL gene)
- HrL1 & rbcL1301RL (for rbcL gene)
- PZatpB1334R-PrL728RL (for atpB-rbcL)
脚注
- ↑ 1.0 1.1 The PCR primer "rbcL-26Fmas" of Table 1 in Masuzaki et al. (2010a) was published with the sequences based on mistranscription of the sequences data, which should be rectified as "5'-TATTAATTGA TTTGTAGGGA GG-3'". In avoiding future confusion we gave a new name to the sequences (rbcL-26Fh: 5'-TATTAATTGA TTTGTAGGGA GG-3').
- ↑ Masuzaki et al.（2011）のHrL911Rの配列は5'-CGTGCAATGC ATGCAGTTAT TGATAGACA-3'となっているが，実際に用いたものはHrL911Rtmの配列．
- ↑ Masuzaki et al.（2011）の配列を訂正
- ↑ Masuzaki et al. (2011)のrbcL600Rの配列は5'-CGTGGTGGAC TTGATTTCAC-3'となっているが，誤り．
- ↑ Inoue et al. (2011)では，Masuzaki et al. (2010)が初出となっているが，実際にはInoue et al. (2011)が初出．
- ↑ The first appearance of this primer was in Table 1 of Inoue et al. (2011), and the footnote in Inoue et al. (2011) should be requested to make the corrections as the following: correct: “(published here)” and incorrect: “published by Masuzaki et al. (2010)”.
References
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関連ページ
- UBC primers
- IUPAC/UBMB codes for nucleic acid bases [Nucleotide base codes (IUPAC)]
- Nucleotide base codes
- pyrimidine
- purine
デジタル自然史博物館 / 広島大学 / 植物 / コケ植物（蘚苔類） / 情報 にもどる
