PCR primers cp rps4-trnT-trnL-trnF

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PCR primers

chloroplast

rps4 (ribosomal protein S4; ribosomal protein subunit 4)

Table. Primers used for PCR amplification and sequencing
Analyzed region Forward / Reverse Primer name Sequence (5'–3') References For
rps4 Forward trnT36R GTAATGCGAT GGTCATCGGT TCGACTCCGA TA Inoue et al. (2012) Bryophytes
rps4 Forward rps5 ATGTCCCGTT ATCGAGGACC T Nadot et al. (1994) Bryophytes
rps4 Forward rps4_1R ATGTCCCGTT ATCGAGGACC TCGTGTA Inoue et al. (2012) Bryophytes
rps4 Forward rps4_2Fmas TGTCCCGTTA TCGAGGACC Masuzaki et al. (2010) Bryophytes
rps4 Forward rps4_19Fi CCTCGTGTAA GAATAATACG TC Inoue & Tsubota (2014) Bryophytes
rps4 Forward rps4_275Fmas AGATAATGTG ATCTTTCGAT TAGGTATGGC Masuzaki et al. (2010) Bryophytes
rps4 Forward rps4_347Fmas CAATTGGTTA ATCATAGACA Masuzaki et al. (2010) Bryophytes
rps4 Forward rps4_553Fmas CAAACGATTA ATCGTGAATG GA Masuzaki et al. (2010) Bryophytes
rps4 Reverse psaA340F CTTGAGCACT AGGTTTAATA TGAGTAGGAT CA Inoue et al. (2012) Bryophytes
rps4 Reverse trnS (syn. trans) TACCGAGGGT TCGAATC Souza-Chies et al. (1997), Buck et al. (2000) Vascular plants (Souza-Chies et al. 1997), Bryophytes (Buck et al. 2000, as trans)
rps4 Reverse rps4_609RL TTAAGCTTGA CGAGAATAAT ATTC Masuzaki et al. (2010) Bryophytes
rps4 Reverse psa3' ATATTCTACA ACTAACAACT C Nadot et al. (1994) Bryophytes
rps4 Reverse rps4_602Fn (psa4_602Fn) TGACGAGAAT AATATTCTAC AACTA Inoue & Tsubota (2014) Bryophytes
rps4 Reverse rps4_605Rmas ACTTGGCGAG AATAATATTC AAC Masuzaki et al. (2010) Bryophytes
rps4 Reverse rps4_578Ri CGAGAATAAT ATTCTACAAC TA Inoue & Tsubota (2014) Bryophytes
rps4 Reverse rps4_531Rmas TCCATTCACG ATTAATCGTT TG Masuzaki et al. (2010) Bryophytes
rps4 Reverse rps4_305Rmas GCCATACCTA ATCGAAAGAT CACATTATCT Masuzaki et al. (2010) Bryophytes

rps4trnTtrnLtrnF

Table. Primers used for PCR amplification and sequencing
Analyzed region Forward / Reverse Primer name Sequence (5'–3') References For
rps4trnTtrnLtrnF Forward rps4_609RL TTAAGCTTGA CGAGAATAAT ATTC Masuzaki et al. (2010) Bryophytes (Masuzaki et al. 2010); Vascular plants (Lysimachia, Tsubota et al. 2015b)
trnTtrnLtrnF Forward trnT71F CCGCTATCGG AGTCGAACCG ATGACCATCG Tsubota et al. (2015b) Vascular plants (Lysimachia, Tsubota et al. 2015b)
trnLtrnF Forward a [trnT(UGU)F-a] CATTACAAAT GCGATGCTCT Taberlet et al. (1991) Vascular plants
trnLtrnF Forward c [trnL(UAA)5exonF-c] CGAAATCGGT AGACGCTACG Taberlet et al. (1991) Vascular plants
trnLtrnF Forward g GGGCAATCCT GAGCCAA Taberlet et al. (2007) Vascular plants
trnLtrnF Forward e [trnL(UAA)3exonF-e] GGTTCAAGTC CCTCTATCCC Taberlet et al. (1991) Vascular plants
rps4trnTtrnLtrnF Reverse trnF1F TGCCAGAAAC CAGATTTGAA CTGGTGACAC Tsubota et al. (2015b) Vascular plants (Lysimachia, Tsubota et al. 2015b)
rps4trnTtrnLtrnF Reverse trnF8F TCAGTCCTCT GCTCTACCAA CTGAGCTA Tsubota et al. (2015b) Vascular plants (Lysimachia, Tsubota et al. 2015b)
trnLtrnF Reverse f [trnF(GAA)R-f] ATTTGAACTG GTGACACGAG Taberlet et al. (1991) Vascular plants
trnLtrnF Reverse d [trnL(UAA)3exonR-d] GGGGATAGAG GGACTTGAAC Taberlet et al. (1991) Vascular plants
trnLtrnF Reverse h CCATTGAGTC TCTGCACCTA TC Taberlet et al. (2007) Vascular plants
trnLtrnF Reverse b [trnL(UAA)5exonR-b] TCTACCGATT TCGCCATATC Taberlet et al. (1991) Vascular plants

Example of primer pairs

  • trnF1F & trnS (for rps4-trnT-trnL-trnF)
  • trnF1F & trnT71F (for trnL-trnF region)
  • trnT36R & trnS (for rps4 gene)

References

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